Zhu Y, Luo K, Wang J
Department of Infectious Diseases, Nanfang Hospital, Guangzhou 510515.
Zhonghua Nei Ke Za Zhi. 1999 Jun;38(6):387-9.
The clearance of intrahepatic hepatitis B virus (HBV) is mainly by the cytotoxicity of immune cells with ways including CD95 ligand/CD95 induction. We found that the liver cells do express CD95 ligand (CD95L), a cytotoxic cell surface molecule. This paper studied the mechanism and implications of the hepatocytes themselves to clear the virus.
The HepG2 cells with induced CD95L and the HepG2.2.15 cells with constitutive CD95 expression were coincubated. The apoptotic cells were examined under fluorescence microscope and with flow cytometry.
In chronic hepatitis B intrahepatic expressions of CD95L and/or CD95 were detected at both mRNA and protein level. Methotrexate induced CD95L expression of HepG2 cell. The HepG2 cells with CD95L could kill the HepG2.2.15 cells with CD95 molecule with apoptotic rates of 16.5% at 24h and 43.0% at 48h. This apoptosis effect could be blocked by using anti-CD95L antibody.
The hepatocyte might induce apoptosis mutually and clear the virus themselves with a nonimmune mechanism.
肝内乙型肝炎病毒（HBV）的清除主要通过免疫细胞的细胞毒性作用，其方式包括CD95配体/CD95诱导。我们发现肝细胞确实表达细胞毒性细胞表面分子CD95配体（CD95L）。本文研究了肝细胞自身清除病毒的机制及意义。
将诱导表达CD95L的HepG2细胞与组成性表达CD95的HepG2.2.15细胞共同培养。在荧光显微镜下及通过流式细胞术检测凋亡细胞。
在慢性乙型肝炎中，肝内CD95L和/或CD95在mRNA和蛋白质水平均有表达。甲氨蝶呤诱导HepG2细胞表达CD95L。表达CD95L的HepG2细胞能够杀伤表达CD95分子的HepG2.2.15细胞，24小时时凋亡率为16.5%，48小时时凋亡率为43.0%。使用抗CD95L抗体可阻断这种凋亡效应。
肝细胞可能通过非免疫机制相互诱导凋亡并自身清除病毒。
