Gao W, Li X, Pei X
Department of Senile Cardiology, General Hospital of PLA, Beijing 100853, China.
Zhonghua Yi Xue Za Zhi. 2001 Jun 10;81(11):677-9.
To observe human beta(2) adrenergic receptors (beta(2)-AR) gene expression in cardiomyocytes transfected by adenoviral vector contain cmv or mlc-2v promoter.
Two adenoviral vectors (Adcmv beta(2) AR, Admlc beta(2) AR) were constructed, in which beta(2)-AR gene is under control of either the ventricle-specific myosin light chain-2 (mlc-2v) or the cmv promoter. The cultured neonate rat ventricular myocytes were infected by two adenoviral vectors, and the beta(2)-AR expression of on cultured neonate rat ventricular myocytes and their ability to potentiate beta-adrenergic signaling were assessed.
The presence of beta(2)-AR mRNA was detected by RT-PCR, and the expression of the beta(2)-AR gene was demonstrated by protein immunoblots. According to a ligand binding assay, the density of beta-AR in the cardiomyocytes infected by Admlc beta(2) AR and Adcmv beta(2) AR was greater than that in the control. Moreover, the latter was higher than the former (234 +/- 6.4 fmol/mg protein vs 153 +/- 5.2 fmol/mg protein) (P < 0.01). It was demonstrated that beta(2)-AR gene significantly increased isoproterenol stimulated cAMP in cardiomyocytes.
The results above demonstrated that human beta(2)-AR gene was expressed in the cardiomyocytes infected by recombinant adenovirus contain cmv or mlc-2v promoter, but both is different.
