Gao W Q, Li X Y, Wu X B, Pei X T, Li L
Department of Cardiology General Hospital of PLA, Beijing 100853, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2001 May;17(2):157-60.
Recombinant adenovirus (rAd) and adeno-associated virus (rAAV) were created, in which beta2-adrenergic receptors (beta2-AR) gene is under control of the cmv promotor, the cultured neonate rat ventricular myocytes were infected by the two vectors, and the expression of beta2-AR on cultured neonate rat ventricular myocytes was assessed.
RT-PCR demonstrated the presence of beta2-AR mRNA, protein immunoblots demonstrated the expression of the beta2-AR gene. According to a ligand binding assay, the density of beta AR in the cardiomyocytes infected by rAd and rAAV had no difference, which was greater than that in the control.
The results above demonstrated that adenovirus vector and AAV vector transfected efficiently cardiomyocytes.
构建重组腺病毒（rAd）和腺相关病毒（rAAV），其中β2 - 肾上腺素能受体（β2 - AR）基因受巨细胞病毒（cmv）启动子调控，用这两种载体感染培养的新生大鼠心室肌细胞，并评估β2 - AR在培养的新生大鼠心室肌细胞上的表达。
逆转录 - 聚合酶链反应（RT - PCR）证实存在β2 - AR信使核糖核酸（mRNA），蛋白质免疫印迹证实β2 - AR基因的表达。根据配体结合试验，受rAd和rAAV感染的心肌细胞中β肾上腺素能受体（βAR）的密度无差异，且高于对照组。
上述结果表明腺病毒载体和腺相关病毒载体能有效转染心肌细胞。
