Nie Q, Zhou Y, Xie Y
The Center of Infectious Disease Diagnosis and Treatment of PLA, Tangdu Hospital, Fourth Military Medical University, Xi'an.710038, China.
Zhonghua Yi Xue Za Zhi. 2001 Jul 10;81(13):805-7.
To investigate the expression and location of tissue inhibitors of metallproteinases-1 and -2 (TIMP-1 and TIMP-2) in liver tissue of patients with cirrhosis and to investigate the correlation between TIMPs in liver and those in serum.
TIMP-1 and TIMP-2 in the serum of 106 patients with liver cirrhosis were detected by solid-phase absorption to sensitized erythrocytes (SPASE). The expression of TIMP-1 and TIMP-2 related antigens in liver biopsy specimens from 30 patients with liver cirrrhosis was detected by immunohistochemical method with specific monoclonal antibody (McAb), and the results thus obtained were compared with the results of the seral TIMPs from the same patients. 10 normal livers were used as controls.
The positive rates of TIMP-1 and TIMP-2 in the 106 serum samples of patients with cirrhosis were 73.58% and 61.32% respectively. TIMP-1 and TIMP-2 related antigens were detected in all of the 30 liver tissues from patients with liver cirrhosis and the expression of TIMP-1 was higher than that of TIMP-2 (P < 0.01). The staining signal of TIMP-1 and TIMP-2 related antigen was mainly located in the cytoplasm of liver cells. There was no signal in nuclear of liver cells. None of the normal liver tissues was positive in expression of TIMP-related antigen. The positive rate was 100% for serum TIMP-1 and 77% for serum TIMP-2.
Expression of TIMPs at different degrees can be found in liver tissue with cirrhosis. TIMPs are located in cytoplasm of liver cells of patients with cirrhosis. There is a significant correlation between serum TIMPs level and liver TIMPs level. Serum TIMP-1 and TIMP-2 can be used as diagnostic indexes for liver fibrosis in chronic liver diseases.
研究金属蛋白酶组织抑制剂-1和-2（TIMP-1和TIMP-2）在肝硬化患者肝组织中的表达及定位，并探讨肝组织与血清中TIMP之间的相关性。
采用致敏红细胞固相吸附法（SPASE）检测106例肝硬化患者血清中的TIMP-1和TIMP-2。应用特异性单克隆抗体（McAb）免疫组化法检测30例肝硬化患者肝活检标本中TIMP-1和TIMP-2相关抗原的表达，并将结果与同一患者的血清TIMP检测结果进行比较。以10例正常肝脏作为对照。
106例肝硬化患者血清中TIMP-1和TIMP-2的阳性率分别为73.58%和61.32%。30例肝硬化患者的肝组织均检测到TIMP-1和TIMP-2相关抗原，且TIMP-1的表达高于TIMP-2（P<0.01）。TIMP-1和TIMP-2相关抗原的染色信号主要位于肝细胞的细胞质中，肝细胞细胞核无信号。正常肝组织TIMP相关抗原表达均为阴性。血清TIMP-1阳性率为100%，血清TIMP-2阳性率为77%。
肝硬化肝组织中可不同程度地检测到TIMP的表达。TIMP定位于肝硬化患者肝细胞的细胞质中。血清TIMP水平与肝组织TIMP水平存在显著相关性。血清TIMP-1和TIMP-2可作为慢性肝病肝纤维化的诊断指标。
