Tan Thean Y
Department of Medical Microbiology and Public Health Laboratory, University Hospital of Wales, Heath Park, Cardiff.
J Med Microbiol. 2002 Jan;51(1):83-5. doi: 10.1099/0022-1317-51-1-83.
Coagulase-negative staphylococci (CNS) are common isolates from blood cultures, and an increasing proportion is now methicillin resistant. The National Committee for Clinical Laboratory Standards (NCCLS) recently issued new criteria for zone sizes applicable to oxacillin disk sensitivity testing for CNS and the British Society of Antimicrobial Chemotherapy (BSAC) has also issued guidelines. This study evaluated two standard methods for oxacillin disk sensitivity testing of 67 CNS isolates from blood cultures, and compared these results with detection of the mecA gene by PCR. Over 94% of mecA-positive isolates were detected by conventional disk testing, with no significant differences between the two methods. In this study, the clinical utility of mecA detection in CNS for the determination of methicillin resistance appears to be limited.
凝固酶阴性葡萄球菌（CNS）是血培养中常见的分离菌，目前耐甲氧西林的比例在增加。美国国家临床实验室标准委员会（NCCLS）最近发布了适用于CNS的苯唑西林纸片药敏试验的新的抑菌圈大小标准，英国抗菌化疗协会（BSAC）也发布了指南。本研究评估了两种用于对67株血培养分离的CNS进行苯唑西林纸片药敏试验的标准方法，并将这些结果与通过聚合酶链反应（PCR）检测mecA基因的结果进行比较。超过94%的mecA阳性分离株通过传统纸片试验检测到，两种方法之间无显著差异。在本研究中，检测CNS中的mecA用于确定甲氧西林耐药性的临床实用性似乎有限。
