Chartogne Anne, Gaspari Marco, Jespersen Sonja, Buscher Brigitte, Verheij Elwin, Heijden Rv Robert van der, Tjaden Ubbo, van der Greef Jan
Division of Analytical Chemistry, Leiden/Amsterdam Center for Drug Research, P.O. Box 9502, 2300 RA Leiden, The Netherlands.
Rapid Commun Mass Spectrom. 2002;16(3):201-7. doi: 10.1002/rcm.566.
In the present work, we describe a collection system for the off-line coupling of capillary isoelectric focusing (CIEF) with matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. In this system, the capillary effluent is directly deposited in fractions onto the MALDI target via the use of a sheath liquid. The collected fractions are subsequently supplemented with matrix and further analysed by MALDI-TOF mass spectrometry for mass assignment. The experimental set-up includes a fiber optic based UV detector operating at 280 nm, which allows the study of the influence of the sheath liquid composition on the CIEF separation. The influence of the carrier ampholyte concentration on the protein MALDI spectra was also evaluated and the feasibility of the collection method was finally demonstrated with a mixture of four standard proteins.
在本工作中，我们描述了一种用于毛细管等电聚焦（CIEF）与基质辅助激光解吸/电离飞行时间（MALDI-TOF）质谱离线联用的收集系统。在该系统中，毛细管流出物通过使用鞘液直接以馏分形式沉积到MALDI靶上。随后，收集的馏分补充基质，并通过MALDI-TOF质谱进一步分析以进行质量归属。实验装置包括一个基于光纤的在280 nm处运行的紫外检测器，它可以研究鞘液组成对CIEF分离的影响。还评估了载体两性电解质浓度对蛋白质MALDI光谱的影响，最终用四种标准蛋白质的混合物证明了该收集方法的可行性。
