基于喷墨技术的 CE-MALDI 接口。
CE-MALDI interface based on inkjet technology.
机构信息
Department of Chemistry, University of Washington, Seattle, WA, USA.
出版信息
Electrophoresis. 2009 Dec;30(23):4071-4. doi: 10.1002/elps.200900414.
Abstract
An ink jet printer valve and a nozzle were used to deliver matrix and sample from an electrophoresis capillary onto a MALDI plate. The system was evaluated by the separation of a set of standard peptides. That separation generated up to 40 000 theoretical plates in less than 3 min. Detection limits were 500 amol for an ABI TOF-TOF instrument and 2 fmol for an ABI Q-TOF instrument. Over 70% coverage was obtained for the tryptic digest of alpha-lactalbumin in less than 2.5 min.
摘要
一种喷墨打印机阀和喷嘴被用于将电泳毛细管中的基质和样品递送到 MALDI 板上。该系统通过一组标准肽的分离进行了评估。该分离在不到 3 分钟的时间内产生了多达 40000 个理论塔板。对于 ABI TOF-TOF 仪器，检测限为 500 amol，对于 ABI Q-TOF 仪器，检测限为 2 fmol。不到 2.5 分钟，就可以获得α-乳白蛋白胰蛋白酶消化物的超过 70%的覆盖率。
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