Al-Harthi Lena, Guilbert Larry J, Hoxie James A, Landay Alan
Department of Immunology/Microbiology, Rush-Presbyterian-St. Luke's Medical Center, Rush University, Chicago, Illinois 60612, USA.
AIDS Res Hum Retroviruses. 2002 Jan 1;18(1):13-7. doi: 10.1089/088922202753394673.
Evidence for HIV-1 infection of trophoblasts is discordant. Utilizing highly purified full-term trophoblasts, we demonstrate that full-term trophoblasts express CXCR4 but are negative for CCR5 and CD4 cell surface proteins. Full-term trophoblasts were refractory to infection by HIV-1 IIIB and primary isolates of HIV-1. However, full-term trophoblasts could be infected by a CD4-independent, CXCR4-utilizing HIV-1 strain, as demonstrated by substantial p24 (5.5 ng/ml) levels and HIV-1 gag/pol DNA content (3050 copies/microg) 7 days postinfection. These data illustrate that trophoblasts express the essential host factors for productive HIV-1 infection and that the block to HIV-1 infection may be at the level of entry. In additional, our data suggest that CD4-independent mechanisms of infection may play a role in promoting in utero HIV-1 transmission.
关于滋养层细胞感染HIV-1的证据并不一致。利用高度纯化的足月滋养层细胞，我们证明足月滋养层细胞表达CXCR4，但CCR5和CD4细胞表面蛋白呈阴性。足月滋养层细胞对HIV-1 IIIB及HIV-1原代分离株的感染具有抗性。然而，如感染7天后p24（5.5纳克/毫升）的显著水平及HIV-1 gag/pol DNA含量（3050拷贝/微克）所示，足月滋养层细胞可被一种不依赖CD4、利用CXCR4的HIV-1毒株感染。这些数据表明，滋养层细胞表达了HIV-1有效感染所需的宿主因子，而对HIV-1感染的阻碍可能发生在病毒进入阶段。此外，我们的数据表明，不依赖CD4的感染机制可能在促进子宫内HIV-1传播中发挥作用。
