Perrier Anselme L, Massoulié Jean, Krejci Eric
Laboratoire de Neurobiologie Cellulaire et Moléculaire, CNRS UMR 8544, Ecole Normale Supérieure, 46 rue d'Ulm, 75230 Paris Cedex 05, France.
Neuron. 2002 Jan 17;33(2):275-85. doi: 10.1016/s0896-6273(01)00584-0.
As a tetramer, acetylcholinesterase (AChE) is anchored to the basal lamina of the neuromuscular junction and to the membrane of neuronal synapses. We have previously shown that collagen Q (ColQ) anchors AChE at the neuromuscular junction. We have now cloned the gene PRiMA (proline-rich membrane anchor) encoding the AChE anchor in mammalian brain. We show that PRiMA is able to organize AChE into tetramers and to anchor them at the surface of transfected cells. Furthermore, we demonstrate that AChE is actually anchored in neural cell membranes through its interaction with PRiMA. Finally, we propose that only PRiMA anchors AChE in mammalian brain and muscle cell membranes.
作为一种四聚体，乙酰胆碱酯酶（AChE）锚定在神经肌肉接头的基膜和神经元突触膜上。我们之前已经表明，胶原蛋白Q（ColQ）在神经肌肉接头处锚定AChE。我们现在已经克隆了编码哺乳动物脑中AChE锚定蛋白的PRiMA（富含脯氨酸的膜锚定蛋白）基因。我们表明，PRiMA能够将AChE组装成四聚体并将它们锚定在转染细胞的表面。此外，我们证明AChE实际上是通过与PRiMA的相互作用而锚定在神经细胞膜上。最后，我们提出，在哺乳动物脑和肌肉细胞膜中，只有PRiMA能锚定AChE。
