人体组织酯酶同工酶的初步遗传学解释。

A preliminary genetic interpretation of the esterase isozymes of human tissues.

Coates P M, Mestriner M A, Hopkinson D A

Ann Hum Genet. 1975 Jul;39(1):1-20. doi: 10.1111/j.1469-1809.1975.tb00103.x.

Abstract

The esterase isozymes of human tissues have been investigated using the technique of starch-gel electrophoresis. Conventional naphthyl-azo dye linked stains and new fluorogenic staining methods were used to detect the isozymes. 2. Multiple isozymes were identified in every tissue and they were characterized in terms of their electrophoretic mobility, tissue distribution, developmental changes in utero, substrate specificity, inhibition properties and molecular weight. On these criteria 13 sets of esterase isozymes were identified, in addition to the esterase isozymes due to cholinesterase and carbonic anhydrase. 3. The data suggest that the 13 sets of isozymes are determined by at least nine different structural gene loci. 4. No electrophoretic variants were identified in a limited population survey of post-mortem tissues from adults and foetuses, except for the previously described esterase D (ESD) phenotypes.

摘要

利用淀粉凝胶电泳技术对人体组织的酯酶同工酶进行了研究。采用传统的萘基偶氮染料连接染色法和新的荧光染色法来检测同工酶。2. 在每个组织中都鉴定出了多种同工酶，并根据它们的电泳迁移率、组织分布、子宫内发育变化、底物特异性、抑制特性和分子量进行了表征。根据这些标准，除了胆碱酯酶和碳酸酐酶产生的酯酶同工酶外，还鉴定出了13组酯酶同工酶。3. 数据表明，这13组同工酶至少由9个不同的结构基因位点决定。4. 在对成人和胎儿的死后组织进行的有限人群调查中，除了先前描述的酯酶D（ESD）表型外，未发现电泳变异体。