人类己糖激酶分布与特性的电泳研究

An electrophoretic study of the distribution and properties of human hexokinases.

作者信息

Rogers P A, Fisher R A, Harris H

出版信息

Biochem Genet. 1975 Dec;13(11-12):857-66. doi: 10.1007/BF00484416.

Abstract

An electrophoretic system which gives a clear separation of human hexokinases HK1, HK2 and HK3 is described. The distribution of the hexokinase isozymes in various human tissues, both adult and fetal, is reported. Some properties of the isozymes were investigated. HK2 was found to be more thermolabile than HK1, and there was also a small but significant difference in molecular size. Unlike HK3, HK1 and HK2 are not inhibited by high glucose concentrations. Screening of red cell lysates from 800 unrelated European individuals revealed no genetic variants of HK1 and HK2. However, in view of their difference in properties, it seems probable that the HK1 and HK2 isozymes are determined by separate gene loci.

摘要

本文描述了一种能清晰分离人己糖激酶HK1、HK2和HK3的电泳系统。报道了己糖激酶同工酶在成人和胎儿各种人体组织中的分布情况。对这些同工酶的一些特性进行了研究。发现HK2比HK1更不耐热，并且在分子大小上也存在微小但显著的差异。与HK3不同，HK1和HK2不受高葡萄糖浓度的抑制。对800名无亲缘关系的欧洲个体的红细胞裂解物进行筛查，未发现HK1和HK2的基因变异。然而，鉴于它们在特性上的差异，HK1和HK2同工酶似乎很可能由不同的基因位点决定。