Bertrand Thomas, Jolivalt Claude, Caminade Eliane, Joly Nathalie, Mougin Christian, Briozzo Pierre
Laboratoire d'Enzymologie et de Biochimie Structurales, UPR 9063 du CNRS, 91198 Gif-sur-Yvette CEDEX, France.
Acta Crystallogr D Biol Crystallogr. 2002 Feb;58(Pt 2):319-21. doi: 10.1107/s0907444901019898. Epub 2002 Jan 24.
Laccases are multi-copper oxidases that catalyse the oxidation of a wide range of phenols and their use in industrial oxidative processes is increasing. A laccase has been purified from the fungus Trametes versicolor and crystallized using the hanging-drop method. Crystals belong to the monoclinic space group P2(1), with unit-cell parameters a = 87.7, b = 110.5, c = 123.2 A, beta = 103.4 degrees. A complete data set was collected to 2.4 A resolution on a Cu K(alpha) rotating-anode X-ray source. Molecular replacement was performed and the initial electron-density maps indicate that the four expected Cu atoms are all present.
漆酶是多铜氧化酶，可催化多种酚类的氧化，其在工业氧化过程中的应用正在增加。已从云芝真菌中纯化出一种漆酶，并采用悬滴法进行了结晶。晶体属于单斜空间群P2(1)，晶胞参数为a = 87.7、b = 110.5、c = 123.2 Å，β = 103.4°。在铜Kα旋转阳极X射线源上收集了完整的数据集，分辨率达到2.4 Å。进行了分子置换，初始电子密度图表明所有四个预期的铜原子均存在。
