Pegasova Tatiana V, Zwart Petrus, Koroleva Olga V, Stepanova Elena V, Rebrikov Denis V, Lamzin Victor S
A. N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky Prospekt 33, 119071 Moscow, Russia.
Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1459-61. doi: 10.1107/s0907444903011521. Epub 2003 Jul 23.
Laccase from the fungus Coriolus hirsutus has been purified. Crystals of the enzyme suitable for X-ray structure analysis have been obtained under optimized crystallization conditions using polyethylene glycol as a precipitant. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 50.65, b = 74.01, c = 124.83 A, and contain 40% solvent and a single molecule of laccase in the asymmetric unit. X-ray data were collected to 1.85 A at the copper edge and the four copper sites have been located from the anomalous signal. The obtained SAD phases with subsequent density modification produced a promising initial electron-density map.
