Mehra Sukvarsha, Messner Hans, Minden Mark, Chaganti R S K
Laboratory of Cancer Genetics, Cell Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.
Genes Chromosomes Cancer. 2002 Mar;33(3):225-34. doi: 10.1002/gcc.10025.
Spectral karyotyping (SKY) and comparative genomic hybridization (CGH) have greatly enhanced the resolution of cytogenetic analysis, enabling the identification of novel regions of rearrangement and amplification in tumor cells. Here we report the analysis of 10 malignant non-Hodgkin lymphoma (NHL) cell lines derived at the Ontario Cancer Institute (OCI), Toronto, designated as OCI-Ly1, OCI-Ly2, OCI-Ly3, OCI-LY4, OCI-Ly7, OCI-Ly8, OCI-Ly12, OCI-Ly13.2, OCI-Ly17, and OCI-Ly18, by G-banding, SKY, and CGH, and we present their comprehensive cytogenetic profiles. In contrast to the 52 breakpoints identified by G-banding, SKY identified 87 breakpoints, which clustered at 1q21, 7p15, 8p11, 13q21, 13q32, 14q32, 17q11, and 18q21. G-banding identified 10 translocations, including the previously described recurring translocations, t(8;14)(q24;q32) and t(14;18)(q32;q21). In contrast, SKY identified 60 translocations, including five that were recurring, t(8;14)(q24;q32), t(14;18)(q32;q21), t(4;7)(p12;q22), t(11;18)(q22;q21), and t(3;18)(q21;p11). SKY also identified the source of all the marker chromosomes. In addition, 10 chromosomes that were classified as normal by G-banding were found by SKY to be rearranged. CGH identified seven sites of high-level DNA amplification, 1q31-32, 2p12-16, 8q24, 11q23-25, 13q21-22, 13q32-34, and 18q21-23; of these, 1q31-32, 11q23-25, 13q21-22, and 13q32-34 have previously not been described as amplified in NHL. This comprehensive cytogenetic characterization of 10 NHL cell lines identified novel sites of rearrangement and amplification; it also enhances their value in experimental studies aimed at gene discovery and gene function.
光谱核型分析（SKY）和比较基因组杂交（CGH）极大地提高了细胞遗传学分析的分辨率，能够识别肿瘤细胞中重排和扩增的新区域。在此，我们报告了对10株源自多伦多安大略癌症研究所（OCI）的恶性非霍奇金淋巴瘤（NHL）细胞系的分析，这些细胞系分别命名为OCI-Ly1、OCI-Ly2、OCI-Ly3、OCI-LY4、OCI-Ly7、OCI-Ly8、OCI-Ly12、OCI-Ly13.2、OCI-Ly17和OCI-Ly18，采用G显带、SKY和CGH技术进行分析，并展示了它们全面的细胞遗传学图谱。与G显带识别出的52个断点相比，SKY识别出87个断点，这些断点聚集在1q21、7p15、8p11、13q21、13q32、14q32、17q11和18q21。G显带识别出10种易位，包括先前描述的常见易位t(8;14)(q24;q32)和t(14;18)(q32;q21)。相比之下，SKY识别出60种易位，包括5种常见易位，即t(8;14)(q24;q32)、t(14;18)(q32;q21)、t(4;7)(p12;q22)、t(11;18)(q22;q21)和t(3;18)(q21;p11)。SKY还识别出所有标记染色体的来源。此外，SKY发现10条被G显带分类为正常的染色体发生了重排。CGH识别出7个高水平DNA扩增位点，分别为1q31-32、2p12-16、8q24、11q23-25、13q21-22、13q32-34和18q21-23；其中，1q31-32、11q23-25、13q21-22和13q32-34此前未被描述为在NHL中发生扩增。这10株NHL细胞系的全面细胞遗传学特征识别出了重排和扩增的新位点；同时也提高了它们在旨在基因发现和基因功能研究的实验中的价值。
