Riede U N, Schmidt E, Kiefer G, Rohrbach R, Sandritter W
Beitr Pathol. 1975 Jul;155(3):263-84. doi: 10.1016/s0005-8165(75)80121-1.
A calcium-rich rachitogenic vitamin-D deficiency and additional doses of calcitonin cause serious changes in cell structure and metabolism, which can be demonstrated by cytophotometry and morphometry. The experiments were made with 9 male juvenile Wistar rats. 6 animals were kept on a vitamin-D deficient diet for 6 weeks and 3 of these animals got injections with salmon-calcitonin S.C. during the last 14 days every second day. The remaining 3 animals, fed with altromin-R standard diet were kept for control purposes only. The morphometric analysis of the liver parenchymal cells was based on the statements by Weibel and the cytophotometric examinations were made according to the Sandritter technique. As a consequence of the altered metabolic condition a great volume reduction of cells and cell nuclei is found in the animals with vitamin-D deficiency, which were treated with calcitonin. In comparison with the control animals the hepatocytes show hardly any tetraploid nuclei, which points to an inhibited polyploidization. The cytoplasm shows an oncocytic transformation with megamitochondria. As reason for this mitochondriopathy an extreme and abnormal growth as well as a mutation of the mitochondrial genom are under discussion. Furthermore the total endoplasmic reticulum (volume and membrane surface), the peroxisomes and the ribosomes are strongly reduced. Due to the serious cell injury the other organelles are also reduced in number and volume, which points to a chronic hypocalcemia.
富含钙的致佝偻病性维生素D缺乏症以及额外剂量的降钙素会导致细胞结构和代谢发生严重变化，这可以通过细胞光度测定法和形态测定法得到证实。实验选用了9只雄性幼年Wistar大鼠。6只动物被给予缺乏维生素D的饮食6周，其中3只动物在最后14天每隔一天皮下注射鲑鱼降钙素。其余3只喂食altromin-R标准饮食的动物仅作为对照。肝实质细胞的形态测定分析基于韦贝尔的描述，细胞光度测定检查则按照桑德里特技术进行。由于代谢状况改变，在缺乏维生素D且接受降钙素治疗的动物中发现细胞和细胞核的体积大幅减小。与对照动物相比，肝细胞几乎没有四倍体核，这表明多倍体化受到抑制。细胞质显示出具有巨型线粒体的嗜酸性细胞转化。关于这种线粒体病的原因，正在讨论线粒体基因组的极端异常生长和突变。此外，总的内质网（体积和膜表面积）、过氧化物酶体和核糖体也大幅减少。由于严重的细胞损伤，其他细胞器的数量和体积也减少，这表明存在慢性低钙血症。
