Kotani S, Watanabe Y, Shimono T, Kinoshita F, Narita T
Biken J. 1975 Jun;18(2):93-103.
Attempts were made to isolate and identify the unit chemical structure essential for manifestation of the immunoadjuvant activities characteristic of bacterial cell walls. The N-acetylmuramyl-peptide subunit monomers, Nalpha-(N-acetylmuramyl-L-alanyl-D-isoglutaminyl)-Nepsilon-(glycylglycyl)-L-lysyl-D-alanine from the cell walls of Staphylococcus aureus (FDA 209P) and N-acetylmuramyl-L-alanyl-D-isoglutaminyl-meso-diaminopimelic acid and/or N-acetylmuramyl-L-alanyl-D-isoglutaminyl-meso-diaminopimelyl-D-alanine from those of Lactobacillus plantarum (ATCC 8014), were shown to be unit chemical entities with definite adjuvant activity both in stimulation of antibody production and in induction of delayed-type hypersensitivity to ovalbumin when administered to guinea-pigs as water-in-oil emulsions.
人们试图分离并鉴定出对于细菌细胞壁所特有的免疫佐剂活性表现至关重要的单位化学结构。来自金黄色葡萄球菌（FDA 209P）细胞壁的N-乙酰胞壁酰肽亚基单体Nα-（N-乙酰胞壁酰-L-丙氨酰-D-异谷氨酰胺基）-Nε-（甘氨酰甘氨酰）-L-赖氨酰-D-丙氨酸，以及来自植物乳杆菌（ATCC 8014）细胞壁的N-乙酰胞壁酰-L-丙氨酰-D-异谷氨酰胺基-内消旋二氨基庚二酸和/或N-乙酰胞壁酰-L-丙氨酰-D-异谷氨酰胺基-内消旋二氨基庚二酰-D-丙氨酸，当作为油包水乳液给豚鼠注射时，在刺激抗体产生以及诱导对卵清蛋白的迟发型超敏反应方面均显示为具有明确佐剂活性的单位化学实体。
