Kotani S, Narita T, Stewart-Tull D E, Shimono T, Watanabe Y
Biken J. 1975 Jun;18(2):77-92.
The cell walls from all 21 species of gram-positive bacteria examined, except lysozyme-susceptible Micrococcus lysodeikticus (NCTC 2665) and lysozyme-resistant Staphylococcus epidermidis (ATCC 155), were found to be definitely adjuvant-active in both stimulation of increased serum antibody levels and induction of delayed-type hypersensitivity to ovalbumin when administered to guinea-pigs as water-in-oil emulsions. Using various cell wall lytic enzymes, the immunoadjuvant principles were solubilized with full retention of the adjuvant activities from walls of Staphylococcus aureus (Copenhagen), Streptococcus pyogens (group A, type 6; S43/100), Streptococcus salivarius (IFO 3350), Streptococcus faecalis (IFO 12580), Streptococcus mutans (BHT), Lactobacillus plantarum (ATCC 8014), Bacillus megaterium (IFO 12068), Corynebacterium diphtheriae (Park-Williams No. 8), Mycobacterium smegmatis, and Actinomyces viscous (ATCC 15987). Evidence was obtained that the non-peptidoglycan portion of the cell walls is not essential for manifestation of immunoadjuvancy.
在所检测的21种革兰氏阳性细菌中，除了对溶菌酶敏感的溶壁微球菌（NCTC 2665）和对溶菌酶耐药的表皮葡萄球菌（ATCC 155）外，当以油包水乳液形式给豚鼠注射时，其余细菌的细胞壁在刺激血清抗体水平升高以及诱导对卵清蛋白的迟发型超敏反应方面均表现出明确的佐剂活性。使用各种细胞壁裂解酶，从金黄色葡萄球菌（哥本哈根株）、化脓性链球菌（A组，6型；S43/100）、唾液链球菌（IFO 3350）、粪肠球菌（IFO 12580）、变形链球菌（BHT）、植物乳杆菌（ATCC 8014）、巨大芽孢杆菌（IFO 12068）、白喉棒状杆菌（帕克-威廉姆斯8号）、耻垢分枝杆菌和黏性放线菌（ATCC 15987）的细胞壁中溶解出了免疫佐剂成分，且完全保留了佐剂活性。有证据表明，细胞壁的非肽聚糖部分对于免疫佐剂活性的表现并非必不可少。
