Thornhill D P
Biochem J. 1975 May;147(2):215-9. doi: 10.1042/bj1470215.
Purified elastin was hydrolysed with HCl and manipulated under conditions that minimized oxidation. Gel-permeation chromatography on polyacrylamide gel and ion-exchange chromatography on dextran cation-exchanger each resulted in the separation of a series of yellow fluorescent fractions. These hitherto unreported ampholytes have fluorescence spectra that approximate to that of the intact protein, and account for its characteristic optical properties. Since the coloured fluorophores are confined to enzyme-resistant regions of the protein molecule they appear to have important structural implications.
纯化的弹性蛋白用盐酸水解，并在使氧化最小化的条件下进行处理。在聚丙烯酰胺凝胶上进行凝胶渗透色谱，以及在葡聚糖阳离子交换剂上进行离子交换色谱，均导致一系列黄色荧光级分的分离。这些迄今未报道的两性电解质具有与完整蛋白质近似的荧光光谱，并解释了其特征光学性质。由于有色荧光团局限于蛋白质分子的酶抗性区域，它们似乎具有重要的结构意义。
