Deyl Z, Macek K, Adam M, Vancíková O
Biochim Biophys Acta. 1980 Oct 21;625(2):248-54. doi: 10.1016/0005-2795(80)90288-3.
Two fluorescent fractions were found in total acid hydrolysate of elastin. The fraction with higher chromatography mobility in isopropyl alcohol/conc. ammonia/water (9 : 1 : 2) was purified by multiple preparative paper chromatography in the same solvent system, and by gel chromatography on Sephadex G-25, ion-exchange chromatography on phosphocellulose and another gel chromatography on Sephadex G-10. The purified material was chromatographically homogeneous, had an ultraviolet absorption maximum at 315 nm and exhibited a strong 320/405 nm fluorescence. 1H- and 13C-NMR spectra were in good agreement with those published previously [6] for pyridinoline, a lysine derived fluorescent compound in collagen. The major part of the fluorescent material present in acid hydrolysate of elastin was always contaminated, even after complex purification procedures. It is concluded that elastin contains several fluorophores, one of which is a cross-linking tricarboxylic amino acid with a pyridinium ring having very probably the structure of 3-(2-amino-2-carboxyethyl)-1-(5-amino-5-carboxy-2-hydroxy-pentyl)-4-(3-amino-3- carboxypropyl)-5-hydroxypyridinium. The position of 2-amino-2-carboxyethyl and 3-amino-3-carboxypropyl residues has not been definitely established and can be interchanged.
在弹性蛋白的总酸水解产物中发现了两个荧光组分。在异丙醇/浓氨水/水（9:1:2）中具有较高色谱迁移率的组分，通过在相同溶剂系统中的多次制备性纸色谱、Sephadex G - 25凝胶色谱、磷酸纤维素离子交换色谱以及另一次Sephadex G - 10凝胶色谱进行纯化。纯化后的物质在色谱上是均一的，在315 nm处有最大紫外吸收，并呈现出强烈的320/405 nm荧光。1H - 和13C - NMR光谱与先前发表的关于吡啶啉（胶原蛋白中一种赖氨酸衍生的荧光化合物）的光谱[6]高度一致。即使经过复杂的纯化程序，弹性蛋白酸水解产物中存在的荧光物质的主要部分仍总是受到污染。得出结论认为，弹性蛋白含有几种荧光团，其中一种是具有吡啶环的交联三羧酸氨基酸，其结构很可能为3 - (2 - 氨基 - 2 - 羧乙基)-1 - (5 - 氨基 - 5 - 羧基 - 2 - 羟基戊基)-4 - (3 - 氨基 - 3 - 羧丙基)-5 - 羟基吡啶鎓。2 - 氨基 - 2 - 羧乙基和3 - 氨基 - 3 - 羧丙基残基的确切位置尚未确定，并且可能相互交换。
