Scawen M D, Ramshaw J A, Boulter D
Biochem J. 1975 May;147(2):343-9. doi: 10.1042/bj1470343.
The amino acid sequence of spinach (Spinacia oleracea L.) plastocyanin was determined. It consists of a single polypeptide chain of 99 residues and has a sequence molecular weight of 10415. The sequence was determined by using a Beckman 890C automatic sequencer and by the dansyl--phenyl isothiocyanate analysis of peptides obtained by the enzymic digestion of purified CNBr fragments. Overlap through the two methionine residues was not shown. Sedimentation equilibrium in the ultracentrifuge gave a molecular weight for spinach plastocyanin of about 9000, in contrast with the value of 21000 reported previously by Katoh et al. (1962).
测定了菠菜（Spinacia oleracea L.）质体蓝素的氨基酸序列。它由一条含99个残基的单多肽链组成，序列分子量为10415。该序列是通过使用贝克曼890C自动测序仪以及对纯化的溴化氰片段酶解得到的肽段进行丹磺酰 - 异硫氰酸苯酯分析来确定的。未显示通过两个甲硫氨酸残基的重叠情况。超速离心机中的沉降平衡给出菠菜质体蓝素的分子量约为9000，这与加藤等人（1962年）先前报道的21000的值形成对比。
