Kelly J, Ambler R P
Biochem J. 1974 Dec;143(3):681-90. doi: 10.1042/bj1430681.
The amino acid sequence of the plastocyanin from the green alga Chlorella fusca was determined. The protein consists of a single polypeptide chain of 98 residues, and was determined by characterization of chymotryptic and thermolysin peptides. The amino acid sequence shows considerable similarity to that of higher plant plastocyanins. The protein contains a single cysteine, and the sequence in the vicinity of this residue is similar to that around the cysteine residue of bacterial azurins. The plastocyanin contains some uncharacterized carbohydrate. Detailed evidence for the sequence of the protein has been deposited as Supplementary Publication SUP 50 036 (17pp., 1 microfiche) at the British Library (Lending Division) (formerly the National Lending Library for Science and Technology), Boston Spa, Yorks. LS23 7BQ, U.K., from whom copies may be obtained on the terms given in Biochem. J. (1973) 131, 5.
测定了来自绿藻小球藻的质体蓝素的氨基酸序列。该蛋白质由一条含98个残基的单多肽链组成,通过对胰凝乳蛋白酶和嗜热菌蛋白酶肽段的表征来确定。其氨基酸序列与高等植物质体蓝素的序列有相当大的相似性。该蛋白质含有一个半胱氨酸,此残基附近的序列与细菌天青蛋白半胱氨酸残基周围的序列相似。质体蓝素含有一些未鉴定的碳水化合物。该蛋白质序列的详细证据已作为补充出版物SUP 50 036(17页,1缩微胶片)存放在英国图书馆(借阅部)(原国家科学技术借阅图书馆),地址为英国约克郡波士顿温泉市LS23 7BQ,可按《生物化学杂志》(1973年)131卷第5期所述条件从该处获取复印件。