[The inhibitory effect of bcl-2 antisense oligodeoxynucleotide/RNA on the growth of cervical carcinoma in vitro].

OBJECTIVE

To study the therapeutic effect of bcl-2 antisense oligodeoxynucleotide (ODN)/RNA human cervical carcinoma in vitro.

METHODS

To transfect bcl-2 antisense ODN/RNA into Hela cells with antisense technology and observe the growth and apoptotic modulation of Hela cells.

RESULTS

The expression of bcl-2 protein and mRNA was higher in Hela cells; the bcl-2 specific antisense ODN significantly decreased bcl-2 protein expression and inhibit the growth of Hela cells. The strongest inhibitory effect was at 24 hours after transfection, the optimal concentration of antisense ODN was 10 micromol/L. The results indicated that the inhibitory effect of bcl-2 antisense ODN on cell growth and survival rate is dose dependent; the recombinant retroviral vector pDOR-AB can transfer antisense bcl-2 gene into the genome of Hela cells, bcl-2 protein and mRNA expressions were significantly decreased after transfected, it demonstrated bcl-2 antisense RNA can significantly inhibit the growth of Hela cells and induce their apoptosis.

CONCLUSION

The results demonstrate the feasibility of bcl-2 gene as a target for antisense gene therapy of human cervical carcinoma and provide the theoretical foundation and experimental evidence for further study in vivo.