DNA聚合酶II在暴露于紫外线前营养应激的紫外线照射大肠杆菌中未切除的胸腺嘧啶二聚体耐受性中的作用。

Role of DNA polymerase II in the tolerance of thymine dimers remaining unexcised in UV-irradiated Escherichia coli exposed to pre-UV nutritional stress.

作者信息

Sedliakova M, Slezarikova V, Masek F, Vizvaryova M, Pirsel M

机构信息

Department of Molecular Genetics, Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Slovak Republic.

出版信息

J Photochem Photobiol B. 2001 Dec 31;65(2-3):145-50. doi: 10.1016/s1011-1344(01)00256-1.

DOI:10.1016/s1011-1344(01)00256-1

PMID:11809372

Abstract

Nutritional stress applied prior to UV-irradiation to E. coli 15 555-7 reduced thymine dimer excision and inhibited post-UV incorporation of thymidine in polB(+) as well as in polB(-) cells. However, the pre-UV-stressed polB(+) cells were significantly more UV-resistant and after UV synthesized larger DNA molecules than the pre-UV-stressed polB(-) cells. The data suggest that DNA polymerase II is involved in the tolerance of unremoved thymine dimers.

摘要

在对大肠杆菌15 555 - 7进行紫外线照射之前施加营养应激，会减少胸腺嘧啶二聚体切除，并抑制胸腺嘧啶核苷在polB(+)和polB(-)细胞中的紫外线照射后掺入。然而，紫外线照射前受到应激的polB(+)细胞比紫外线照射前受到应激的polB(-)细胞对紫外线的抵抗力明显更强，并且在紫外线照射后合成的DNA分子更大。数据表明，DNA聚合酶II参与了未去除的胸腺嘧啶二聚体的耐受性。