鉴定与DNA复制起点重合的人类复制起点识别复合体蛋白1和2的结合区域。
Identification of a binding region for human origin recognition complex proteins 1 and 2 that coincides with an origin of DNA replication.
作者信息
Ladenburger Eva-Maria, Keller Christian, Knippers Rolf
机构信息
Department of Biology, Universität Konstanz, D-78464 Konstanz, Germany.
出版信息
Mol Cell Biol. 2002 Feb;22(4):1036-48. doi: 10.1128/MCB.22.4.1036-1048.2002.
Abstract
We investigated the binding regions of components of the origin recognition complex (ORC) in the human genome. For this purpose, we performed chromatin immunoprecipitation assays with antibodies against human Orc1 and Orc2 proteins. We identified a binding region for human Orc proteins 1 and 2 in a <1-kbp segment between two divergently transcribed human genes. The region is characterized by CpG tracts and a central sequence rich in AT base pairs. Both, Orc1 and Orc2 proteins are found at the intergenic region in the G(1) phase, but S-phase chromatin contains only Orc2 protein, supporting the notion that Orc1p dissociates from its binding site in the S phase. Sequences corresponding to the intergenic region are highly abundant in a fraction of nascent DNA strands, strongly suggesting that this region not only harbors the binding sites for Orc1 protein and Orc2 protein but also serves as an origin of bidirectional DNA replication.
摘要
我们研究了人类基因组中起始识别复合物（ORC）各组分的结合区域。为此，我们用针对人类Orc1和Orc2蛋白的抗体进行了染色质免疫沉淀试验。我们在两个反向转录的人类基因之间的一个<1千碱基对的片段中确定了人类Orc蛋白1和2的一个结合区域。该区域的特征是富含CpG序列以及一个富含AT碱基对的中心序列。在G1期，Orc1和Orc2蛋白都存在于基因间区域，但S期染色质中仅含有Orc2蛋白，这支持了Orc1p在S期从其结合位点解离的观点。与该基因间区域相对应的序列在一部分新生DNA链中高度丰富，这强烈表明该区域不仅含有Orc1蛋白和Orc2蛋白的结合位点，还作为双向DNA复制的起点。
相似文献
1
Identification of a binding region for human origin recognition complex proteins 1 and 2 that coincides with an origin of DNA replication.鉴定与DNA复制起点重合的人类复制起点识别复合体蛋白1和2的结合区域。
Mol Cell Biol. 2002 Feb;22(4):1036-48. doi: 10.1128/MCB.22.4.1036-1048.2002.
2
Localization of proteins bound to a replication origin of human DNA along the cell cycle.细胞周期中与人类DNA复制起点结合的蛋白质的定位
EMBO J. 2003 Aug 15;22(16):4294-303. doi: 10.1093/emboj/cdg404.
3
Human Mcm proteins at a replication origin during the G1 to S phase transition.在G1期到S期转变过程中，人类微小染色体维持（Mcm）蛋白位于复制起点处。
Nucleic Acids Res. 2002 Oct 1;30(19):4176-85. doi: 10.1093/nar/gkf532.
4
The origin recognition complex marks a replication origin in the human TOP1 gene promoter.起始识别复合物标记人类TOP1基因启动子中的一个复制起点。
J Biol Chem. 2002 Aug 30;277(35):31430-40. doi: 10.1074/jbc.M202165200. Epub 2002 May 9.
5
Human minichromosome maintenance proteins and human origin recognition complex 2 protein on chromatin.染色质上的人类微小染色体维持蛋白和人类起源识别复合体2蛋白。
J Biol Chem. 1998 Sep 18;273(38):24543-9. doi: 10.1074/jbc.273.38.24543.
6
Differential binding of replication proteins across the human c-myc replicator.复制蛋白在人类c-myc复制起点上的差异结合
Mol Cell Biol. 2006 Jul;26(14):5270-83. doi: 10.1128/MCB.02137-05.
7
Nuclear organization of DNA replication initiation proteins in mammalian cells.哺乳动物细胞中DNA复制起始蛋白的核组织。
J Biol Chem. 2002 Mar 22;277(12):10354-61. doi: 10.1074/jbc.M111398200. Epub 2002 Jan 4.
8
Phosphorylation of ORC2 protein dissociates origin recognition complex from chromatin and replication origins.磷酸化 ORC2 蛋白将起始识别复合物从染色质和复制起点上分离。
J Biol Chem. 2012 Apr 6;287(15):11891-8. doi: 10.1074/jbc.M111.338467. Epub 2012 Feb 13.
9
The ORC1 cycle in human cells: II. Dynamic changes in the human ORC complex during the cell cycle.人类细胞中的ORC1循环：II. 细胞周期中人类ORC复合物的动态变化。
J Biol Chem. 2003 Oct 17;278(42):41535-40. doi: 10.1074/jbc.M307535200. Epub 2003 Aug 8.
10
ORC, MCM, and histone hyperacetylation at the Kaposi's sarcoma-associated herpesvirus latent replication origin.卡波西肉瘤相关疱疹病毒潜伏复制起点处的ORC、MCM与组蛋白高度乙酰化
J Virol. 2004 Nov;78(22):12566-75. doi: 10.1128/JVI.78.22.12566-12575.2004.
引用本文的文献
1
Origins of DNA replication in eukaryotes.真核生物中 DNA 复制的起源。
Mol Cell. 2023 Feb 2;83(3):352-372. doi: 10.1016/j.molcel.2022.12.024. Epub 2023 Jan 13.
2
Human ORC/MCM density is low in active genes and correlates with replication time but does not delimit initiation zones.人类 ORC/MCM 密度在活跃基因中较低，与复制时间相关，但不限制起始区域。
Elife. 2021 Mar 8;10:e62161. doi: 10.7554/eLife.62161.
3
A predictable conserved DNA base composition signature defines human core DNA replication origins.可预测的保守 DNA 碱基组成特征定义了人类核心 DNA 复制起点。
Nat Commun. 2020 Sep 21;11(1):4826. doi: 10.1038/s41467-020-18527-0.
4
Genome-wide identification and characterisation of human DNA replication origins by initiation site sequencing (ini-seq).通过起始位点测序（ini-seq）对人类DNA复制起点进行全基因组鉴定和表征。
Nucleic Acids Res. 2016 Dec 1;44(21):10230-10247. doi: 10.1093/nar/gkw760. Epub 2016 Sep 1.
5
Selectivity of ORC binding sites and the relation to replication timing, fragile sites, and deletions in cancers.ORC结合位点的选择性及其与复制时间、脆性位点和癌症中缺失的关系。
Proc Natl Acad Sci U S A. 2016 Aug 16;113(33):E4810-9. doi: 10.1073/pnas.1609060113. Epub 2016 Jul 19.
6
Cdk1-mediated phosphorylation of Cdc7 suppresses DNA re-replication.细胞周期蛋白依赖性激酶1（Cdk1）介导的细胞分裂周期蛋白7（Cdc7）磷酸化可抑制DNA再复制。
Cell Cycle. 2016 Jun 2;15(11):1494-505. doi: 10.1080/15384101.2016.1176658. Epub 2016 Apr 22.
7
Mapping of Replication Origins in the X Inactivation Center of Vole Microtus levis Reveals Extended Replication Initiation Zone.田鼠X染色体失活中心复制起点的定位揭示了扩展的复制起始区
PLoS One. 2015 Jun 3;10(6):e0128497. doi: 10.1371/journal.pone.0128497. eCollection 2015.
8
Human DNA helicase B interacts with the replication initiation protein Cdc45 and facilitates Cdc45 binding onto chromatin.人类DNA解旋酶B与复制起始蛋白Cdc45相互作用，并促进Cdc45与染色质的结合。
Exp Cell Res. 2015 Jun 10;334(2):283-93. doi: 10.1016/j.yexcr.2015.04.014. Epub 2015 Apr 29.
9
Allele-specific genome-wide profiling in human primary erythroblasts reveal replication program organization.人类原代红细胞中全基因组等位基因特异性分析揭示了复制程序的组织方式。
PLoS Genet. 2014 May 1;10(5):e1004319. doi: 10.1371/journal.pgen.1004319. eCollection 2014 May.
10
High-resolution analysis of DNA synthesis start sites and nucleosome architecture at efficient mammalian replication origins.高效哺乳动物复制起始点处 DNA 合成起始位点和核小体结构的高分辨率分析。
EMBO J. 2013 Oct 2;32(19):2631-44. doi: 10.1038/emboj.2013.195. Epub 2013 Aug 30.
本文引用的文献
1
Plaque formation and isolation of pure lines with poliomyelitis viruses.脊髓灰质炎病毒的噬斑形成及纯系分离
J Exp Med. 1954 Feb;99(2):167-82. doi: 10.1084/jem.99.2.167.
2
Architecture of the human origin recognition complex.人类起源识别复合体的结构
J Biol Chem. 2001 Aug 3;276(31):29067-71. doi: 10.1074/jbc.M103078200. Epub 2001 Jun 6.
3
Assembly of the human origin recognition complex.人类起始识别复合体的组装
J Biol Chem. 2001 Jul 13;276(28):26666-73. doi: 10.1074/jbc.M102493200. Epub 2001 Apr 25.
4
Where it all starts: eukaryotic origins of DNA replication.一切的起点：DNA复制的真核起源
J Cell Sci. 2001 Feb;114(Pt 4):643-51. doi: 10.1242/jcs.114.4.643.
5
Genome-wide location and function of DNA binding proteins.DNA结合蛋白的全基因组定位与功能
Science. 2000 Dec 22;290(5500):2306-9. doi: 10.1126/science.290.5500.2306.
6
The human origin recognition complex protein 1 dissociates from chromatin during S phase in HeLa cells.人类起源识别复合体蛋白1在HeLa细胞的S期从染色质上解离。
J Biol Chem. 2001 Mar 2;276(9):6337-42. doi: 10.1074/jbc.M009473200. Epub 2000 Dec 1.
7
SV40 large T antigen functions in DNA replication and transformation.SV40大T抗原在DNA复制和转化过程中发挥作用。
Adv Virus Res. 2000;55:75-134. doi: 10.1016/s0065-3527(00)55002-7.
8
Chromatin association of human origin recognition complex, cdc6, and minichromosome maintenance proteins during the cell cycle: assembly of prereplication complexes in late mitosis.细胞周期中人类起源识别复合体、细胞分裂周期蛋白6及微小染色体维持蛋白与染色质的关联：有丝分裂后期复制前复合体的组装
Mol Cell Biol. 2000 Nov;20(22):8602-12. doi: 10.1128/MCB.20.22.8602-8612.2000.
9
Identification and characterization of the human ORC6 homolog.人类ORC6同源物的鉴定与表征
J Biol Chem. 2000 Nov 10;275(45):34983-8. doi: 10.1074/jbc.M006069200.
10
Selective instability of Orc1 protein accounts for the absence of functional origin recognition complexes during the M-G(1) transition in mammals.Orc1蛋白的选择性不稳定导致哺乳动物在M期向G1期转变过程中缺乏功能性的复制起始识别复合体。
EMBO J. 2000 Jun 1;19(11):2728-38. doi: 10.1093/emboj/19.11.2728.
Zotero 插件