hMutSα在DNA上与hMutLα和hMutLβ形成一种依赖ATP的复合物。
hMutSalpha forms an ATP-dependent complex with hMutLalpha and hMutLbeta on DNA.
作者信息
Plotz Guido, Raedle Jochen, Brieger Angela, Trojan Jörg, Zeuzem Stefan
机构信息
Second Department of Medicine, Johann Wolfgang Goethe-University, Theodor Stern-Kai 7, D-60590 Frankfurt am Main, Germany.
出版信息
Nucleic Acids Res. 2002 Feb 1;30(3):711-8. doi: 10.1093/nar/30.3.711.
Abstract
The DNA binding properties of hMutSalpha and hMutLalpha and complex formation of hMutSalpha with hMutLalpha and hMutLbeta were investigated using binding experiments on magnetic bead-coupled DNA substrates with nuclear extracts as well as purified proteins. hMutSalpha binding to homoduplex DNA was disrupted by lower NaCl concentrations than hMutSalpha binding to a mismatch. ATP markedly reduced the salt resistance of hMutSalpha binding but hMutSalpha still retained affinity for heteroduplexes. hMutSalpha formed a complex with hMutLalpha and hMutLbeta on DNA in the presence of ATP. This complex only formed on 81mer and not 32mer DNA substrates. Complex formation was enhanced by a mismatch in the DNA substrate, and hMutLalpha and hMutLbeta were shown to enter the complex at different ATP concentrations. Purified hMutLalpha showed an intrinsic affinity for DNA, with a preference for single-stranded over double-stranded DNA.
摘要
利用磁珠偶联的DNA底物与核提取物以及纯化蛋白进行结合实验，研究了hMutSα和hMutLα的DNA结合特性以及hMutSα与hMutLα和hMutLβ的复合物形成。与hMutSα结合错配相比，较低的NaCl浓度即可破坏hMutSα与同型双链DNA的结合。ATP显著降低了hMutSα结合的耐盐性，但hMutSα对异源双链体仍保持亲和力。在ATP存在的情况下，hMutSα在DNA上与hMutLα和hMutLβ形成复合物。这种复合物仅在81聚体DNA底物上形成，而不在32聚体DNA底物上形成。DNA底物中的错配增强了复合物的形成，并且显示hMutLα和hMutLβ在不同的ATP浓度下进入复合物。纯化的hMutLα对DNA表现出内在亲和力，对单链DNA的偏好超过双链DNA。
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