Wu Li, Bashirova Arman A, Martin Thomas D, Villamide Loreley, Mehlhop Erin, Chertov Andrei O, Unutmaz Derya, Pope Melissa, Carrington Mary, KewalRamani Vineet N
HIV Drug Resistance Program, Laboratory of Genomic Diversity, and Basic Research Program, Science Applications International Corporation Frederick, National Cancer Institute, National Institutes of Health, Frederick, MD 21702, USA.
Proc Natl Acad Sci U S A. 2002 Feb 5;99(3):1568-73. doi: 10.1073/pnas.032654399. Epub 2002 Jan 29.
Here, we describe the isolation and characterization of the rhesus macaque homolog for human DC-SIGN, a dendritic cell-specific C-type lectin. mac-DC-SIGN is 92% identical to hu-DC-SIGN. mac-DC-SIGN preserves the virus transmission function of hu-DC-SIGN, capturing and efficiently transducing simian and human immunodeficiency virus to target CD4(+) T cells. Surprisingly, however, mac-DC-SIGN plays no discernable role in the ability of rhesus macaque dendritic cells to capture and transmit primate lentiviruses. Expression and neutralization analyses suggest that this process is DC-SIGN independent in macaque, although the participation of other lectin molecules cannot be ruled out. The ability of primate lentiviruses to effectively use human and rhesus dendritic cells in virus transmission without the cells becoming directly infected suggests that these viruses have taken advantage of a conserved dendritic cell mechanism in which DC-SIGN family molecules are significant contributors but not the only participants.
在此，我们描述了人类树突状细胞特异性C型凝集素DC-SIGN的恒河猴同源物的分离与特性。恒河猴DC-SIGN（mac-DC-SIGN）与人类DC-SIGN（hu-DC-SIGN）有92%的同源性。mac-DC-SIGN保留了hu-DC-SIGN的病毒传播功能，能够捕获并有效地将猿猴免疫缺陷病毒和人类免疫缺陷病毒传递给靶CD4(+) T细胞。然而，令人惊讶的是，mac-DC-SIGN在恒河猴树突状细胞捕获和传递灵长类慢病毒的能力中没有可识别的作用。表达和中和分析表明，在猕猴中这个过程不依赖于DC-SIGN，尽管不能排除其他凝集素分子的参与。灵长类慢病毒能够在不直接感染细胞的情况下有效利用人类和恒河猴树突状细胞进行病毒传播，这表明这些病毒利用了一种保守的树突状细胞机制，其中DC-SIGN家族分子是重要贡献者但不是唯一参与者。
