Alfa Michelle J, Ilnyckyj Alexandra, MacFarlane Nila, Preece Vivienne, Allford Sandra, Fachnie Beth
Department of Medical Microbiology, University of Manitoba, Canada.
Gastrointest Endosc. 2002 Feb;55(2):209-13. doi: 10.1067/mge.2002.121339.
There are few data on microbial levels in water used during the assessment of GI motility. Patients undergoing such procedures may be ingesting water with unacceptably high levels of bacteria.
Samples of water from the reservoir and tubing from water perfusion motility equipment were taken and quantitatively assessed to determine the concentration of viable aerobic and facultative microorganisms. Interventions were evaluated to determine which reprocessing schedule ensures absence of overgrowth by microbes within the system during storage.
Bacterial overgrowth can occur in manometry systems with bacterial levels of greater than 10(4) colony-forming units (cfu)/mL in the water from both the reservoir and the tubing. Organisms detected included Serratia marcescens, Burkholderia species, and other gram-negative nonfermentors. Eradication of these organisms was difficult, and the only intervention that consistently ensured bacterial water levels below 200 cfu/mL (i.e., within potable water guidelines) was retrofitting of the pump/tubing with new components combined with a monthly hydrogen peroxide decontamination protocol and a daily drying protocol.
The entire tubing path of motility equipment must be stored dry to prevent microbial overgrowth. Additionally, implementation of a motility equipment quality assurance program with water testing 3 to 4 times per year is recommended to ensure that overgrowth is not a problem.
关于胃肠动力评估过程中所用水的微生物水平的数据很少。接受此类检查的患者可能会摄入细菌含量高得不可接受的水。
采集水灌注动力设备的储水器和管道中的水样，并进行定量评估，以确定需氧和兼性活微生物的浓度。对干预措施进行评估，以确定哪种再处理方案可确保系统在储存期间不会出现微生物过度生长。
测压系统中可能会发生细菌过度生长，储水器和管道中的水中细菌水平均大于10⁴ 菌落形成单位（cfu）/mL。检测到的微生物包括粘质沙雷氏菌、伯克霍尔德菌属以及其他革兰氏阴性非发酵菌。根除这些微生物很困难，唯一能始终确保细菌水含量低于200 cfu/mL（即符合饮用水标准）的干预措施是用新部件对泵/管道进行改装，并结合每月一次的过氧化氢去污方案和每日干燥方案。
动力设备的整个管道路径必须保持干燥储存，以防止微生物过度生长。此外，建议实施动力设备质量保证计划，每年对水进行3至4次检测，以确保不存在过度生长问题。
