Wang Xing-Wang, Xie Hong
World J Gastroenterol. 1998 Dec;4(6):540-543. doi: 10.3748/wjg.v4.i6.540.
To study the expression of Fas and Bcl-2 proteins in BEL-7404 human hepatoma cells in order to analyze the possible relationship between cell growth regulation by alpha-fetoprotein(AFP) and Fas/Bcl-2 proteins.METHODS:BEL-7404 human hepatoma cells were maintained in RPMI 1640 medium supplemented with 10% new-born calf serum. Cells adhered to coverslips were used to detect Fas and Bcl-2 protein expression by the avidin-biotin complex (ABC) immunocytochemical assay.RESULTS: Immunocytochemical study showed that essentially all the BEL-7404 human hepatoma cells could express Fas and Bcl-2 proteins, although in various amount. No positive staining for Fas and Bcl-2 proteins was observed when cells were incubated with non-relevant sera, to establish the specificity.CONCLUSION:Fas apoptosis signals and Bcl-2 rescue/survival signals from apoptosis are expressed in BEL-7404 human hepatoma cells. The finding strongly implys that AFP-mediated cell apoptosis and growth enhancement are potentially associated with Fas and Bcl-2 proteins present in those cells.
研究Fas和Bcl-2蛋白在人BEL-7404肝癌细胞中的表达，以分析甲胎蛋白（AFP）对细胞生长的调控与Fas/Bcl-2蛋白之间可能存在的关系。方法：人BEL-7404肝癌细胞培养于添加10%新生牛血清的RPMI 1640培养基中。采用抗生物素蛋白-生物素复合物（ABC）免疫细胞化学分析法检测贴壁于盖玻片上的细胞中Fas和Bcl-2蛋白的表达。结果：免疫细胞化学研究显示，基本上所有的人BEL-7404肝癌细胞均可表达Fas和Bcl-2蛋白，不过表达量各不相同。为确定特异性，当细胞与无关血清孵育时，未观察到Fas和Bcl-2蛋白的阳性染色。结论：Fas凋亡信号以及Bcl-2从凋亡中拯救/存活信号在人BEL-7404肝癌细胞中表达。这一发现强烈提示，AFP介导的细胞凋亡和生长增强可能与这些细胞中存在的Fas和Bcl-2蛋白有关。
