伏格列波糖和匙羹藤酸对麦芽糖体内吸收的抑制作用。

Inhibitory effect of voglibose and gymnemic acid on maltose absorption in vivo.

作者信息

Luo H, Imoto T, Hiji Y

机构信息

Department of Physiology, Institute of Basic Medicine, CAMS/PUMC,5 Dongdansantiao Beijing 100005, China.

出版信息

World J Gastroenterol. 2001 Apr;7(2):270-4. doi: 10.3748/wjg.v7.i2.270.

DOI:10.3748/wjg.v7.i2.270

PMID:11819773

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4723535/

Abstract

AIM

To determine whether diabetic care can be improved by combination of voglibose and gymnemic acid (GA), we compared the combinative and individual effects of voglibose and GA on maltose absorption in small intestine.

METHODS

The small intestine 30 cm long from 2 cm caudal ward Treitz's ligament of Wistar rat was used as an in situ loop, which was randomly perfused in recircular mode with maltose (10mmol/L) with or without different dosages of voglibose and/or GA for an hour. To compare the time course, perfusion of 10 mmol/L maltose was repeated four times. Each time continued for 1 hour and separated by 30 minutes rinse. In the first time, lower dosages of GA (0.5g/L) and/or voglibose (2 micromol/L) were contained except control.

RESULTS

Absorptive rate of maltose was the lowest in combinative group (P<0.05, ANOVA), for example, the inhibition rate was about 37% during the first hour when 0.5 g/L-GA and 2 micromol/L voglibose with 10 mmol/L maltose were perfused in the loop. The onset time was shortened to 30 minutes and the effective duration was prolonged to 4 hours with the combination; therefore the total amount of maltose absorption during the effective duration was inhibited more significantly than that in the individual administration (P < 0.05, U test of Mann Whitney). The effect of GA on absorptive barriers of the intestine played an important role in the combinative effects.

CONCLUSION

There are augmented effects of voglibose and GA. The management of diabetes mellitus can be improved by employing the combination.

摘要

目的

为了确定伏格列波糖和匙羹藤酸（GA）联合使用是否能改善糖尿病护理，我们比较了伏格列波糖和GA对小肠麦芽糖吸收的联合作用和单独作用。

方法

将来自Wistar大鼠Treitz韧带尾侧2 cm处30 cm长的小肠作为原位肠袢，以再循环模式随机灌注含或不含不同剂量伏格列波糖和/或GA的麦芽糖（10 mmol/L）1小时。为比较时间进程，重复灌注4次10 mmol/L麦芽糖。每次持续1小时，间隔30分钟冲洗。第一次时，除对照组外，含有较低剂量的GA（0.5 g/L）和/或伏格列波糖（2 μmol/L）。

结果

联合组麦芽糖吸收率最低（P<0.05，方差分析），例如，当在肠袢中灌注0.5 g/L-GA、2 μmol/L伏格列波糖和10 mmol/L麦芽糖时，第1小时的抑制率约为37%。联合使用时起效时间缩短至30分钟，有效持续时间延长至4小时；因此，有效持续时间内麦芽糖的总吸收量比单独给药时受到更显著的抑制（P < 0.05，Mann-Whitney U检验）。GA对肠道吸收屏障的作用在联合作用中起重要作用。