α-葡萄糖苷酶抑制剂的抑制作用因其来源而异。

Inhibitory effect of alpha-glucosidase inhibitors varies according to its origin.

作者信息

Oki T, Matsui T, Osajima Y

机构信息

Department of Food Science and Technology, Faculty of Agriculture, Kyushu University, , Fukuoka 812-8581, Japan.

出版信息

J Agric Food Chem. 1999 Feb;47(2):550-3. doi: 10.1021/jf980788t.

DOI:10.1021/jf980788t

PMID:10563931

Abstract

The inhibitory effect of alpha-glucosidase (AGH) inhibitors against its origins (baker's yeast and rat, rabbit, and pig small intestines) was investigated. All inhibitors used in this study showed quite different inhibitory activities according to AGH origins. Voglibose, acarbose and glucono-1,5-lactone strongly inhibited mammalian AGHs, whereas no or less inhibition was observed in yeast AGH. On the contrary, (+)-catechin, a good inhibitor against yeast AGH (IC(50) = 1.3 x 10(-)(1) mM) as well as voglibose (IC(50) = 2.6 x 10(-)(2) mM), did not retard the mammalian AGH activity. Subsequent inhibition study with various food components revealed that all of foods except for green (IC(50) = 0.735 mg/mL) and oolong teas (IC(50) = 1.34 mg/mL) showed no inhibitory activity against rat AGH, whereas they inhibited yeast AGH. Consequently, the magnitude of AGH inhibition was greatly affected by its origin, and more attention relating to AGH origin would be needed to evaluate in vitro AGH inhibitory effect.

摘要

研究了α-葡萄糖苷酶（AGH）抑制剂对其来源（面包酵母以及大鼠、兔子和猪的小肠）的抑制作用。本研究中使用的所有抑制剂根据AGH的来源显示出截然不同的抑制活性。伏格列波糖、阿卡波糖和葡萄糖酸-1,5-内酯强烈抑制哺乳动物的AGH，而对酵母AGH未观察到抑制作用或抑制作用较弱。相反，（+）-儿茶素是酵母AGH的良好抑制剂（IC50 = 1.3×10-1 mM）以及伏格列波糖（IC50 = 2.6×10-2 mM），但它不会抑制哺乳动物AGH的活性。随后对各种食物成分的抑制研究表明，除绿茶（IC50 = 0.735 mg/mL）和乌龙茶（IC50 = 1.34 mg/mL）外，所有食物对大鼠AGH均无抑制活性，但它们能抑制酵母AGH。因此，AGH抑制的程度受其来源的极大影响，在评估体外AGH抑制作用时需要更多关注AGH的来源。