Shenoy Rashmi S, Prashanth K V Harish, Manonmani H K
Department of Food Protectants and Infestation Control, CSIR-Central Food Technological Research Institute, Mysore 570020, India.
Academy of Scientific and Innovative Research, CSIR-Central Food Technological Research Institute, Mysore 570020, India.
Evid Based Complement Alternat Med. 2018 Aug 8;2018:7154702. doi: 10.1155/2018/7154702. eCollection 2018.
A triterpene glycoside (TG) fraction isolated and purified from ethanolic extract of (EEGS) was investigated for blood glucose control benefit using methods. The HPLC purified active fraction TG was characterized using FTIR, LC-MS, and NMR. The purified fraction (TG) exhibited effective inhibition of yeast -glucosidase, sucrase, maltase, and pancreatic -amylase with IC values 3.16 ± 0.05 g/mL, 74.07 ± 0.51, 5.69 ± 0.02, and 1.17 ± 0.24 g/mL, respectively, compared to control. TG was characterized to be a mixture of triterpene glycosides: gymnemic acids I, IV, and VII and gymnemagenin. o studies were performed using mouse pancreatic -cell lines (MIN6). TG did not exhibit any toxic effects on -cell viability and showed protection against HO induced ROS generation. There was up to 1.34-fold increase in glucose stimulated insulin secretion (p<0.05) in a dose-dependent manner relative to standard antidiabetic drug glibenclamide. Also, there was further one-fold enhancement in the expression of GLUT2 compared to commercial standard DAG (deacylgymnemic acid). Thus, the present study highlights the effective isolation and therapeutic potential of TG, making it a functional food ingredient and a safe nutraceutical candidate for management of diabetes.
从[植物名称]乙醇提取物（EEGS）中分离纯化得到的三萜糖苷（TG）组分，采用[具体方法]研究其对血糖控制的益处。通过傅里叶变换红外光谱（FTIR）、液相色谱 - 质谱联用（LC - MS）和核磁共振（NMR）对高效液相色谱（HPLC）纯化的活性组分TG进行表征。纯化后的组分（TG）对酵母α - 葡萄糖苷酶、蔗糖酶、麦芽糖酶和胰腺α - 淀粉酶表现出有效抑制作用，与对照组相比，其半数抑制浓度（IC）值分别为3.16±0.05μg/mL、74.07±0.51、5.69±0.02和1.17±0.24μg/mL。TG被鉴定为三萜糖苷的混合物：匙羹藤酸I、IV和VII以及匙羹藤皂苷元。使用小鼠胰腺β - 细胞系（MIN6）进行了两项研究。TG对β - 细胞活力未表现出任何毒性作用，并对过氧化氢（HO）诱导的活性氧（ROS）生成具有保护作用。相对于标准抗糖尿病药物格列本脲，葡萄糖刺激的胰岛素分泌在剂量依赖性方式下增加了1.34倍（p<0.05）。此外，与商业标准去酰基匙羹藤酸（DAG）相比，葡萄糖转运蛋白2（GLUT2）的表达进一步提高了一倍。因此，本研究突出了TG的有效分离及其治疗潜力，使其成为糖尿病管理的功能性食品成分和安全的营养保健品候选物。
