Wang Q, Lin Z Y, Feng X L
Department of Microbiology, Medical Center of Fudan University, Shanghai 200032, China.
World J Gastroenterol. 2001 Jun;7(3):335-9. doi: 10.3748/wjg.v7.i3.335.
To demonstrate the relationship between H-ras oncogene and hepatocellular carcinoma (HCC) metastasis.
Activated H-ras oncogene was transfected into SMMC 7721, a cell line derived from human HCC, by calcium phosphate transfection method. Some metastasis-related parameters were detected in vitro, including adhesion assay, migration assay, expression of collagenase IV(c IV ase) and epidermal growth factor receptor (EGFR).
The abilities of H-ras-transfected cell clones in adhesion to laminin (LN) or fibronectin (FN), migration, c IV ase secretion increased markedly, and the expression of EGFR elevated moderately. More importantly, these alterations were consistent positively with the expression of p21, the protein product of H-ras oncogene.
H-ras oncogene could induce the metastatic phenotype of HCC cell in vitro to raise its metastatic potential.
探讨H-ras癌基因与肝细胞癌（HCC）转移之间的关系。
采用磷酸钙转染法将激活的H-ras癌基因转染至人肝癌细胞系SMMC 7721。体外检测一些与转移相关的参数，包括黏附试验、迁移试验、IV型胶原酶（c IV ase）和表皮生长因子受体（EGFR）的表达。
转染H-ras的细胞克隆对层粘连蛋白（LN）或纤连蛋白（FN）的黏附能力、迁移能力、c IV ase分泌显著增加，EGFR表达适度升高。更重要的是，这些改变与H-ras癌基因的蛋白产物p21的表达呈正相关。
H-ras癌基因可在体外诱导肝癌细胞的转移表型，提高其转移潜能。
