多西他赛对LS174T细胞VEGF、bFGF和MMPs表达的抑制作用。
Inhibitory effects of docetaxel on expression of VEGF, bFGF and MMPs of LS174T cell.
作者信息
Guo Xue-Liang, Lin Geng-Jin, Zhao Hong, Gao Yong, Qian Li-Ping, Xu San-Rong, Fu Li-Na, Xu Qing, Wang Jie-Jun
机构信息
Department of Gastroenterology, Huashan Hospital, Fudan University, Shanghai 200032, China.
出版信息
World J Gastroenterol. 2003 Sep;9(9):1995-8. doi: 10.3748/wjg.v9.i9.1995.
AIM
To study the effects of non-cytotoxic concentrations of docetaxel on some important angiogenic factors of LS174T Cells.
METHODS
The non-cytotoxic concentration of docetaxel and the activity of gelatinase were determined with MTT and gelatin zymography respectively, the expression of VEGF(vascular endothelial growth factor), bFGF (basic fibroblast growth factor), MMP (matrix metalloproteinase) 2 and MMP 9 was investigated with RT-PCR and Western blot.
RESULTS
The maximum non-cytotoxic concentration of docetaxel on LS174T Cells was 1.0 ng/ml. Compared with the solvent control group, 0.1, 0.5, 1.0 ng/ml of docetaxel could downregulate the expression of VEGF, bFGF, MMP 2 and MMP 9 and suppress the activity of gelatinase.
CONCLUSION
Our study suggests that the non-cytotoxic concentrations of docetaxel have strong antiangiogenic activity on LS174T Cells, which suggests docetaxel may be a promising antiangiogenic agent.
目的
研究非细胞毒性浓度的多西他赛对LS174T细胞某些重要血管生成因子的影响。
方法
分别采用MTT法和明胶酶谱法测定多西他赛的非细胞毒性浓度和明胶酶活性，采用RT-PCR和蛋白质印迹法检测血管内皮生长因子（VEGF）、碱性成纤维细胞生长因子（bFGF）、基质金属蛋白酶（MMP）2和MMP 9的表达。
结果
多西他赛对LS174T细胞的最大非细胞毒性浓度为1.0 ng/ml。与溶剂对照组相比，0.1、0.5、1.0 ng/ml的多西他赛可下调VEGF、bFGF、MMP 2和MMP 9的表达，并抑制明胶酶活性。
结论
我们的研究表明，非细胞毒性浓度的多西他赛对LS174T细胞具有较强的抗血管生成活性，这表明多西他赛可能是一种有前景的抗血管生成药物。
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