Hall A, Knowles J R
Biochemistry. 1975 Sep 23;14(19):4348-53. doi: 10.1021/bi00690a032.
By a combination of methods involving enzyme-catalyzed reactions and classical iodination techniques it has been possible to obtain all the relevant rate constants for the uncatalyzed interconversion of dihydroxyacetone phosphate and D-glyceraldehyde 3-phosphate via their common enediol intermediate. These rate constants are compared with those for the individual steps of the triosephosphate isomerase catalyzed reaction, and a quantitative picture of the effectiveness of the enzyme as a catalyst has been delineated. It is apparent that the enzyme increases the enolization rate of dihydroxyacetone phosphate by a factor of more than 10(9) over that of the uncatalyzed reaction.
通过结合酶催化反应和经典碘化技术等多种方法，得以获取磷酸二羟丙酮和3-磷酸-D-甘油醛通过其共同的烯二醇中间体进行非催化相互转化的所有相关速率常数。将这些速率常数与磷酸丙糖异构酶催化反应各个步骤的速率常数进行比较，从而描绘出该酶作为催化剂有效性的定量图景。很明显，该酶使磷酸二羟丙酮的烯醇化速率比非催化反应提高了超过10⁹倍。
