Reznick A Z, Winzler R J
Biochim Biophys Acta. 1975 Oct 9;404(2):268-73. doi: 10.1016/0304-4165(75)90333-5.
Three glycoprotein bands were identified by polyacrylamide disc gel electrophoresis in the perchloric acid soluble fraction of ascitic fluid of Ehrlich ascites tumor in mice. The three proteins were first separated by a new discontinuous preparative electrophoresis apparatus described previously [1]. They were further purified on Sephadex G-100 and then were subjected to chemical characterization. These glycoproteins were rich in glutamic and aspartic acids and contained the sugar moieties galactose, mannose, fucose, N-acetyl-D-glucosamine and sialic acid. The percent sugar composition ranged from 17.7-37.3% of the total weights of these glycoproteins.
通过聚丙烯酰胺圆盘凝胶电泳，在小鼠艾氏腹水瘤腹水的高氯酸可溶性组分中鉴定出三条糖蛋白带。这三种蛋白质首先通过先前描述的新型不连续制备电泳装置进行分离[1]。它们在葡聚糖凝胶G-100上进一步纯化，然后进行化学表征。这些糖蛋白富含谷氨酸和天冬氨酸，并含有半乳糖、甘露糖、岩藻糖、N-乙酰-D-葡萄糖胺和唾液酸等糖基部分。糖的组成百分比占这些糖蛋白总重量的17.7 - 37.3%。
