Reznick A Z, Winzler R J
Biochim Biophys Acta. 1976 Apr 23;428(2):441-4. doi: 10.1016/0304-4165(76)90052-0.
Three glycoproteins, designated as F, M and S glycoproteins were identified in the HCIO4-soluble fraction of ascitic fluid of Ehrlich ascites tumor by 8% polyacrylamide disc gel electrophoresis. They were separated and purified as described previously (Reznick, A.Z. and Winzler, R.J. (1973) Fed. Proc. 32, 368 and Reznick, A.Z., Allen, H.J. and Winzler, R.J. (1973) Anal. Biochem. 52, 395-401) and subjected to physical characterization. Several physical properties such as molecular weights, sedimentation and diffusion coefficients, partial specific volumes, Stoke's radii and frictional ratios were determined. The physical parameters of F and S glycoproteins resemble data that have been reported for orosomucoid and haptoglobin-like glycoproteins, respectively. Properties of M glycoprotein could not be associated with a known glycoprotein.
通过8%聚丙烯酰胺圆盘凝胶电泳，在艾氏腹水瘤腹水的高氯酸可溶性组分中鉴定出三种糖蛋白，分别命名为F、M和S糖蛋白。如前所述（雷兹尼克，A.Z.和温兹勒，R.J.（1973年）《联邦程序》32，368；雷兹尼克，A.Z.、艾伦，H.J.和温兹勒，R.J.（1973年）《分析生物化学》52，395 - 401），它们被分离和纯化，并进行了物理特性分析。测定了几种物理性质，如分子量、沉降和扩散系数、偏比容、斯托克斯半径和摩擦比。F和S糖蛋白的物理参数分别类似于已报道的类粘蛋白和触珠蛋白样糖蛋白的数据。M糖蛋白的性质与已知的糖蛋白无关。
