Scamman J P, Zawacki J K, McMurrich B J, Babior B M
Biochim Biophys Acta. 1975 Oct 9;404(2):281-8. doi: 10.1016/0304-4165(75)90335-9.
The acid glycosidase content of rat liver Kupffer cells was compared with that of hepatocytes and resident peritoneal macrophages. Homogenates of all these cells were able to hydrolyze the p-nitrophenyl glycosides of N-acetylglucosamine, N-acetylgalactosamine, glucose, galactose, fucose and mannose, but not xylose. Activity was greatest against the N-acetylglucosaminoside. With Kupffer cell homogenates, most of the glycosidases behaved as if they were lysosomal enzymes. When expressed as rates of hydrolysis per 10(6) cells, activities against a given substrate by homogenates from the three cell types generally agreed within a factor of 2-4. Significant differences between cell types were found, however, when ratios of glycosidase activities were compared. Furthermore, even though the quantity of glycosidase per cell was similar in Kupffer cells and hepatocytes, the glycosidase concentrations were much higher in the former cells, since Kupffer cells are much smaller than hepatocytes.
将大鼠肝脏库普弗细胞的酸性糖苷酶含量与肝细胞和常驻腹膜巨噬细胞的酸性糖苷酶含量进行了比较。所有这些细胞的匀浆都能够水解N-乙酰葡糖胺、N-乙酰半乳糖胺、葡萄糖、半乳糖、岩藻糖和甘露糖的对硝基苯基糖苷，但不能水解木糖。对N-乙酰葡糖胺糖苷的活性最强。对于库普弗细胞匀浆，大多数糖苷酶表现得好像它们是溶酶体酶。当以每10(6)个细胞的水解速率表示时，三种细胞类型的匀浆对给定底物的活性通常在2至4倍的范围内一致。然而，当比较糖苷酶活性的比率时，发现细胞类型之间存在显著差异。此外，尽管库普弗细胞和肝细胞中每细胞的糖苷酶量相似，但由于库普弗细胞比肝细胞小得多，前者细胞中的糖苷酶浓度要高得多。
