人类肝脏中溶酶体酶的年龄依赖性变化
Age-dependent variations of lysosomal enzymes in human liver.
作者信息
Minami R, Sato S, Kudoh T, Oyanagi K, Nakao T
出版信息
Tohoku J Exp Med. 1979 Sep;129(1):65-70. doi: 10.1620/tjem.129.65.
The specific activities, the Km values, and the elution patterns on DEAE 52 and Sephadex G-150 columns of six lysosomal enzymes in human liver during development were studied. The levels of total beta-D-glucuronidase and N-acetyl-beta-D-glucosaminidase activities in childhood liver were higher than those in fetal liver. The Km values of beta-D-glucuronidase and beta-D-galactosidase in fetal liver were about ten times higher than those in childhood liver. The elution patterns on DEAE 52 of beta-D-glucuronidase and on Sephadex G-150 of alpha-D- and beta-D-galactosidases changed with aging. It is suggested that lysosomal enzymes related to degradation of gangliosides and glycosaminoglycans change during development.
研究了人类肝脏发育过程中六种溶酶体酶的比活性、Km值以及在DEAE 52和Sephadex G - 150柱上的洗脱模式。儿童肝脏中总β - D - 葡萄糖醛酸酶和N - 乙酰 - β - D - 氨基葡萄糖苷酶的活性水平高于胎儿肝脏。胎儿肝脏中β - D - 葡萄糖醛酸酶和β - D - 半乳糖苷酶的Km值约为儿童肝脏的十倍。β - D - 葡萄糖醛酸酶在DEAE 52上的洗脱模式以及α - D - 和β - D - 半乳糖苷酶在Sephadex G - 150上的洗脱模式随年龄而变化。提示与神经节苷脂和糖胺聚糖降解相关的溶酶体酶在发育过程中发生变化。
Zotero 插件