羊水细胞培养中糖苷酶的分子形式与活性
Molecular forms and activities of glycosidases in cultures of amniotic-fluid cells.
作者信息
Hultberg B, Lindsten J, Sjöblad S
出版信息
Biochem J. 1976 Jun 1;155(3):599-605. doi: 10.1042/bj1550599.
Abstract
Ion-exchange chromatography of gel filtration demonstrated the presence of different molecular forms of nine lysosomal enzymes in cultured amniotic-fluid cells. The patterns of molecular forms were similar to those known from skin fibroblasts and liver tissue. During cultivation total enzyme activities fluctuated with the number of passages, without any consistent trend of increase or decrease, and without correlation to the dominating cell type in the culture.
摘要
凝胶过滤离子交换色谱法证明，在培养的羊水细胞中存在九种溶酶体酶的不同分子形式。分子形式的模式与皮肤成纤维细胞和肝脏组织中已知的模式相似。在培养过程中，总酶活性随传代次数波动，没有任何一致的增加或减少趋势，且与培养物中占主导地位的细胞类型无关。
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