[蛙皮素诱导的急性胰腺炎中胰腺微循环障碍的特征]
[The feature of pancreatic microcirculatory impairment in caerulein induced acute pancreatitis].
作者信息
Zhou Z, Zhang Z, Yan L, Shu Y, Cheng Z, Zhao J, Lan P, Feng X, Wang R
机构信息
Department of Hepato-Bili-Pancreato-Surgery, First University Hospital, West China University of Medical Sciences, Chengdu 610041.
出版信息
Zhonghua Wai Ke Za Zhi. 1999 Mar;37(3):138-40, 9.
OBJECTIVE
To study the feature of pancreatic microcirculatory impairment in caerulein-induced acute pancreatitis.
METHODS
The AP model of subcutaneous injection of caerulein was studied by intravital fluorescence microscopy of erythrocytes labeled by FITC (FITC-RBC) and by scanning electron microscopy of vascular corrosion casts and light microscopy of Chinese ink-injected/cleared tissues.
RESULTS
Animals treated with caerulein showed hyper-amylases. Contractions of intralobular arteriolar sphincter, presence of vacuoles in all the layers of sphincter, gross irregularity in capillary network of acini were observed in caerulein-induced groups. The decrease of pancreatic capillary blood flow (P < 0.01), reduction of functional capillary density, and irregular intermittent perfusion (P < 0.05) were found.
CONCLUSIONS
Impairment and contraction of pancreatic intralobular arteriolar sphincter are the initial microcirculatory lesions in the early phase of acute pancreatitis induced by Caerulein, and play a key role in the pancreatic ischemia and pancreatic microvascular failure in acute pancreatitis.
目的
研究蛙皮素诱导的急性胰腺炎中胰腺微循环障碍的特征。
方法
通过用异硫氰酸荧光素标记红细胞（FITC-RBC）的活体荧光显微镜检查、血管铸型扫描电子显微镜检查以及墨汁灌注/清除组织的光学显微镜检查，对皮下注射蛙皮素的急性胰腺炎模型进行研究。
结果
用蛙皮素处理的动物显示淀粉酶水平升高。在蛙皮素诱导组中观察到小叶内小动脉括约肌收缩、括约肌各层出现空泡、腺泡毛细血管网络明显不规则。发现胰腺毛细血管血流量减少（P<0.01）、功能性毛细血管密度降低以及不规则间歇性灌注（P<0.05）。
结论
胰腺小叶内小动脉括约肌的损伤和收缩是蛙皮素诱导的急性胰腺炎早期的初始微循环病变，在急性胰腺炎的胰腺缺血和胰腺微血管功能障碍中起关键作用。
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