大鼠实验性急性胰腺炎中的胰腺微循环障碍
Pancreatic microcirculatory impairment in experimental acute pancreatitis in rats.
作者信息
Zhou Zong-Guang, Chen You-Dai, Sun Wei, Chen Zhong
机构信息
III Department of General Surgery (Gastroenteric Surgery), West China Hospital, Sichuan University, Chengdu 610041, Sichuan, China.
出版信息
World J Gastroenterol. 2002 Oct;8(5):933-6. doi: 10.3748/wjg.v8.i5.933.
AIM
To study the feature of pancreatic microcirculatory impairment, especially the initial changes, in caerulein-induced experimental acute pancreatitis (AP).
METHODS
The pancreatic microcirculation of caerulein-induced AP model was studied by intravital fluorescence microscopy with FITC-labeled erythrocytes (FITC-RBC), scanning electron microscopy of vascular corrosion casts, and light microscopy of Chinese ink-injected/cleared tissues.
RESULTS
Animals in caerulein-treated group showed hyperamylemia (X2), pancreatic oedema, infiltration of inflammatory cells in pancreas. Constrictions of intralobular arteriolar sphincters, presence of vacuoles in all layers of sphincter, and gross irregularity in capillary network of acini were found in the AP specimens. The decrease of pancreatic capillary blood flow (0.34+/-0.10 nl x min(-1) vs 0.91+/-0.06 nl x min(-1) of control, P<0.001), reduction of functional capillary density(277+/-13 cm(-1) vs 349+/-8 cm(-1) of control, P<0.001), and irregular intermittent perfusion were observed in caerulein-induced groups.
CONCLUSION
Impairment and constriction of pancreatic intralobular arteriolar sphincter are the initial microcirculatory lesions in the early phase of acute pancreatitis, and play a key role in the pancreatic ischaemia and pancreatic microvascular failure in acute pancreatitis.
目的
研究在蛙皮素诱导的实验性急性胰腺炎（AP）中胰腺微循环障碍的特征，尤其是初始变化。
方法
采用活体荧光显微镜观察用异硫氰酸荧光素标记红细胞（FITC-RBC）的蛙皮素诱导的AP模型的胰腺微循环，用血管铸型扫描电子显微镜和墨汁灌注/清除组织的光学显微镜进行研究。
结果
蛙皮素治疗组动物出现高淀粉酶血症（X2）、胰腺水肿、胰腺炎症细胞浸润。在AP标本中发现小叶内小动脉括约肌收缩、括约肌各层出现空泡以及腺泡毛细血管网络严重不规则。观察到蛙皮素诱导组胰腺毛细血管血流量减少（0.34±0.10 nl×min⁻¹ 对比对照组的0.91±0.06 nl×min⁻¹，P<0.001）、功能性毛细血管密度降低（277±13 cm⁻¹ 对比对照组的349±8 cm⁻¹，P<0.001）以及不规则间歇性灌注。
结论
胰腺小叶内小动脉括约肌的损伤和收缩是急性胰腺炎早期的初始微循环病变，在急性胰腺炎的胰腺缺血和胰腺微血管功能障碍中起关键作用。
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