血清中肌型烯醇化酶的免疫学测定作为心肌梗死的诊断试验。

Immunological determination of muscle-type enolase in the serum as a diagnostic test for myocardial infarction.

作者信息

Herraez-Dominguez M V, Goldberg D M, Greaves M, Fleming J S, Rider C C, Taylor C B

出版信息

Clin Chim Acta. 1975 Nov 3;64(3):307-15. doi: 10.1016/0009-8981(75)90359-9.

Abstract

An antiserum raised in the chicken against purified rat skeletal muscle enolase neutralized 86% of the enolase activity of human heart muscle and 8.7% +/- 4.8% (mean +/- S.D.) of the enolase activity in normal human serum. The fraction of enolase inhibited by the antiserum, designated as 'immunologically reacting muscle enolase', promptly rose after myocardial infarction in the serum of affected subjects, reaching peak values in excess of 40% in all but one of 23 subjects. Only one subject with ischaemic heart disease, but without infarction, and 2 with miscellaneous conditions, among 117 such patients tested, yielded comparable values. The test appears to be highly specific for myocardial infarction when muscle disease can be excluded and would not be difficult to introduce as a laboratory routine.

摘要

用鸡制备的抗纯化大鼠骨骼肌烯醇化酶抗血清，可中和86%的人心肌烯醇化酶活性以及正常人类血清中8.7%±4.8%（均值±标准差）的烯醇化酶活性。被该抗血清抑制的烯醇化酶部分，即“免疫反应性肌肉烯醇化酶”，在心肌梗死患者血清中于发病后迅速升高，23例患者中除1例外，其余患者的峰值均超过40%。在117例接受检测的此类患者中，只有1例患有缺血性心脏病但无梗死，以及2例患有其他病症的患者呈现出类似数值。当可排除肌肉疾病时，该检测对心肌梗死似乎具有高度特异性，且作为实验室常规检测引入并不困难。