Kuroiwa T, Kajimoto Y, Ohta T
Department of Neurosurgery, Osaka Medical College, Takatsuki City, Osaka, Japan.
Minim Invasive Neurosurg. 2001 Dec;44(4):240-2. doi: 10.1055/s-2001-19929.
Visualization of near-infrared fluorescence through a surgical microscope can provide intraoperative information about deep-seated tissues. We studied the possibility of taking a near-infrared image through a surgical microscope after intravenous injection of indocyanine green (ICG). The surgical microscopes we used were Zeiss models OPMI MD and OPMI CS-NC. We used a halogen lamp as a light source, a band pass filter of 760 - 810 nm as an excitation filter, a laser light of 800 nm as an external light source, and a band pass filter of 820 - 920 nm as a barrier filter. Near-infrared fluorescence of vessels on the brain surface and of the dural sinus was visualized through the dura mater after intravenous injection of 25 mg ICG using a Hitachi KP-160 camera. Near-infrared fluorescence of vessels on the brain surface was observed through the dura mater. Venous images were clearer than were arterial ones. The dural sinus and the bridging veins were also clearly visualized. These results suggest that a clinical application of near-infrared fluorescence microscopy is possible. However, a stronger fluorescence emitted from ICG will be necessary for such a microscope to have practical use.
通过手术显微镜可视化近红外荧光可提供有关深部组织的术中信息。我们研究了静脉注射吲哚菁绿（ICG）后通过手术显微镜获取近红外图像的可能性。我们使用的手术显微镜是蔡司OPMI MD和OPMI CS - NC型号。我们使用卤素灯作为光源，760 - 810 nm的带通滤光片作为激发滤光片，800 nm的激光作为外部光源，820 - 920 nm的带通滤光片作为阻挡滤光片。使用日立KP - 160相机在静脉注射25 mg ICG后，通过硬脑膜观察到脑表面血管和硬脑膜窦的近红外荧光。通过硬脑膜观察到脑表面血管的近红外荧光。静脉图像比动脉图像更清晰。硬脑膜窦和桥静脉也清晰可见。这些结果表明近红外荧光显微镜在临床上具有应用的可能性。然而，对于这种显微镜的实际应用而言，需要ICG发出更强的荧光。
