Fischer Thomas, Gemeinhardt Ines, Wagner Susanne, Stieglitz Dorothee V, Schnorr Jörg, Hermann Kay-Geert A, Ebert Bernd, Petzelt Diethard, Macdonald Rainer, Licha Kai, Schirner Michael, Krenn Veit, Kamradt Thomas, Taupitz Matthias
Department of Radiology, Charité, Universitätsmedizin Berlin, Campus Charité Mitte, Schumannstrasse 20/21, 10098 Berlin, Germany.
Acad Radiol. 2006 Jan;13(1):4-13. doi: 10.1016/j.acra.2005.07.010.
The aim of the study is to evaluate in vivo fluorescence imaging of experimental inflammatory joint disease by applying two different near-infrared (NIR) dyes in a model of Borrelia-induced Lyme arthritis.
Forty mice, 20 with Lyme arthritis and 20 controls, were examined. Two nonspecific NIR carbocyanine dyes, indocyanine green (ICG) and a hydrophilic carbocyanine derivative (1,1'-bis-[4-sulfobutyl] indotricarbocyanine-5,5'-dicarboxylic acid diglucamide monosodium salt [SIDAG]), were administered intravenously at two doses. Fluorescence images were acquired before and during 120 seconds after injection of cyanine dyes. For both dyes, the area under the curve (AUC) was determined for the interval between 40 and 80 seconds after injection. In addition, the slope of the signal decrease was compared among animal groups. Results were compared with histological findings.
The general temporal fluorescence intensity course for ICG was characterized by a rapid increase, with a peak at 40-50 seconds followed by a decrease; conversely for SIDAG, by a slow increase. AUC analysis for both dyes showed that the fluorescence signal differed significantly between controls and arthritic animals (P < .05). Within these groups, there were significant differences between the two doses investigated. ICG differed significantly between control and arthritic animals in the slope of the signal decrease for both doses investigated (P < .05). Histological examination showed early stages of inflammation in arthritic animals.
NIR fluorescence imaging based on the pharmacokinetic behavior of ICG or SIDAG is a promising approach to detect inflammatory joint changes of experimental arthritis. Moreover, SIDAG is suited to differentiate inflammatory and noninflammatory joints 24 hours after dye application.
本研究旨在通过在伯氏疏螺旋体诱导的莱姆关节炎模型中应用两种不同的近红外（NIR）染料，评估实验性炎性关节疾病的体内荧光成像。
对40只小鼠进行检查，其中20只患有莱姆关节炎，20只为对照。静脉注射两种非特异性NIR花青染料，吲哚菁绿（ICG）和亲水性花青衍生物（1,1'-双-[4-磺丁基]吲哚三碳菁-5,5'-二羧酸二葡糖酰胺一钠盐[SIDAG]），采用两种剂量。在注射花青染料前及注射后120秒内采集荧光图像。对于两种染料，均测定注射后40至80秒间隔内的曲线下面积（AUC）。此外，比较各动物组信号下降的斜率。将结果与组织学检查结果进行比较。
ICG的一般时间荧光强度变化特点是快速上升，在40 - 50秒达到峰值后下降；相反，SIDAG是缓慢上升。两种染料的AUC分析表明，对照组和患关节炎动物之间的荧光信号存在显著差异（P < .05）。在这些组中，所研究的两种剂量之间存在显著差异。对于所研究的两种剂量，ICG在对照组和患关节炎动物之间信号下降的斜率存在显著差异（P < .05）。组织学检查显示患关节炎动物处于炎症早期。
基于ICG或SIDAG药代动力学行为的近红外荧光成像，是检测实验性关节炎炎性关节变化的一种有前景的方法。此外，SIDAG适用于在染料应用24小时后区分炎性和非炎性关节。
