婴儿利什曼原虫克隆株的体外和体内感染性之间的相关性

Correlation between in vitro and in vivo infectivity of Leishmania infantum clones.

作者信息

Méndez S, Fernández-Pérez F J, Santín M, De La Fuente C, Cuquerella M, Gómez-Muñoz M T, Alunda J M

机构信息

National Institute of Allergy and Infectious Disease, The National Institutes of Health, Bethesda, Maryland 20892, USA.

出版信息

J Eukaryot Microbiol. 2001 Nov-Dec;48(6):616-21. doi: 10.1111/j.1550-7408.2001.tb00200.x.

DOI:10.1111/j.1550-7408.2001.tb00200.x

PMID:11831769

Abstract

Eleven clones of a single strain of Leishmania infantum (MCAN/ES/88/ISS441, Doba) were analyzed for biological behavior in vivo and in vitro. Different clones showed differences in growth dependent upon the two culture media employed. All clones displayed only slight differences in H2O2 and NaNO2 sensitivity compared to the original strain, whereas in vitro infectivity for mouse peritoneal macrophages differed significantly among the clones. In vivo infections in hamsters correlated strongly with in vitro infectivity. The phenotypic differences found suggest a polyclonal structure for the Leishmania infantum strain studied.

摘要

对婴儿利什曼原虫单一菌株（MCAN/ES/88/ISS441，多巴）的11个克隆进行了体内和体外生物学行为分析。不同克隆在依赖于所使用的两种培养基的生长方面表现出差异。与原始菌株相比，所有克隆在对过氧化氢和亚硝酸钠的敏感性上仅表现出轻微差异，而各克隆对小鼠腹腔巨噬细胞的体外感染性则有显著差异。仓鼠体内感染与体外感染性密切相关。所发现的表型差异表明所研究的婴儿利什曼原虫菌株具有多克隆结构。