Méndez S, Fernández-Pérez F J, de la Fuente C, Cuquerella M, Gómez-Muñoz M T, Alunda J M
Departamento de Patología Animal I (Sanidad Animal), Facultad de Veterinaria, Universidad Complutense de Madrid, Spain.
Parasitol Res. 1999 Jun;85(6):507-9. doi: 10.1007/s004360050587.
Leishmania infantum stationary-phase promastigotes could acquire infectivity via preincubation in a partially anaerobic medium (95% air/5% CO2) for 16 h before the infection, whereas promastigotes were efficiently destroyed when no CO2 was present. Incubation of L. infantum promastigotes with additional glucose (20 and 50 mM) greatly increased infection parameters in the absence of CO2; this is consistent with a "reverse Pasteur effect." Results showed that culture at 33 degrees C permitted survival and amastigote multiplication (a nearly 10-fold increase in amastigotes as compared with those observed in 37 degrees C cultures). This finding was obtained with the two strains of L. infantum tested (Doba and PB75).
婴儿利什曼原虫静止期前鞭毛体在感染前于部分厌氧培养基（95%空气/5%二氧化碳）中预孵育16小时可获得感染性，而当不存在二氧化碳时，前鞭毛体被有效破坏。在不存在二氧化碳的情况下，用额外的葡萄糖（20和50 mM）孵育婴儿利什曼原虫前鞭毛体极大地增加了感染参数；这与“反向巴斯德效应”一致。结果表明，在33℃培养可使虫体存活并使无鞭毛体增殖（与在37℃培养中观察到的相比，无鞭毛体增加近10倍）。在用测试的两株婴儿利什曼原虫（多巴和PB75）中均获得了这一发现。
