Méndez S, Nell M, Alunda J M
Dpto de Patología Animal I, Fac. Veterinaria, Universidad Complutense de Madrid, Spain.
Int J Parasitol. 1996 Jun;26(6):619-22. doi: 10.1016/0020-7519(96)00037-9.
Leishmania infantum promastigotes in axenic culture exhibit limited infectivity for mouse peritoneal macrophages (M phi) in vitro using standard culture conditions (37 degrees C; 95% air/5% CO2) compared to Leishmania donovani promastigotes which induce notable infections. The infectivity of logarithmic (log) and stationary (stat) phase promastigotes of L. infantum was enhanced by the addition of fresh homologous serum, but no amastigotes were observed after 4 days. Prolonged infections, including transformation and survival of intracellular amastigotes in BALB/c mouse and hamster resident peritoneal M phi and M phi cell line J774.G8 were obtained by incubating M phi for 48 h at 26 degrees C prior to standard culture. Enhanced infectivity was observed in a number of L. infantum strains subject to this transient thermal change.
与能引发显著感染的杜氏利什曼原虫前鞭毛体相比，在体外标准培养条件（37℃；95%空气/5%二氧化碳）下，无细胞培养基中培养的婴儿利什曼原虫前鞭毛体对小鼠腹腔巨噬细胞（M phi）的感染力有限。添加新鲜的同源血清可增强婴儿利什曼原虫对数期（log）和稳定期（stat）前鞭毛体的感染力，但4天后未观察到无鞭毛体。通过在标准培养前将巨噬细胞在26℃下孵育48小时，在BALB/c小鼠和仓鼠常驻腹腔巨噬细胞以及巨噬细胞系J774.G8中获得了包括细胞内无鞭毛体的转化和存活在内的长时间感染。在经历这种短暂热变化的许多婴儿利什曼原虫菌株中观察到感染力增强。
