Sun C, Wang Z, Wang Y
Department of Oral and Maxillofacial Surgery, the First Hospital, China Medical University, Shenyang 110001, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2000 Jan;35(1):72-4.
To explore the relationship between protein kinase C (PKC) and invasion/metastasis of salivary adenoid cystic carcinoma and to explore the possibility of treatment against invasion/metastasis of the tumor cell by PKC inhibitor.
SACC-83 cell line cells were treated by endogenous PKC inhibitor BS-PKCI extracted from bovine spermatozoa and exogenous PKC inhibitor H(7), while the adhesion to basement membrane, movement (expressed by the cell numbers moving out from agarose drop and the furthest distance) and invasive capacity in vitro were observed.
(1) The two inhibitors suppressed significantly adhesion of the tumor cell to basement membrane (P < 0.05). (2) The two inhibitors suppressed significantly movement of the tumor cells (P < 0.05). (3) The two inhibitors suppressed significantly invasive capacity of the tumor cells in vitro (P < 0.01).
There was a close relationship between PKC and invasion/metastasis of salivary adenoid cystic carcinoma; the two PKC inhibitor suppressed significantly adhesion, movement and invasive capacity of SACC - 83 cell line cells in vitro.
探讨蛋白激酶C（PKC）与涎腺腺样囊性癌侵袭转移的关系，以及PKC抑制剂对肿瘤细胞侵袭转移的治疗作用。
采用从牛精子中提取的内源性PKC抑制剂BS - PKCI和外源性PKC抑制剂H(7)处理SACC - 83细胞系细胞，观察其对基底膜的黏附、运动（以从琼脂糖滴中移出的细胞数和最远移动距离表示）及体外侵袭能力。
（1）两种抑制剂均显著抑制肿瘤细胞对基底膜的黏附（P < 0.05）。（2）两种抑制剂均显著抑制肿瘤细胞的运动（P < 0.05）。（3）两种抑制剂均显著抑制肿瘤细胞的体外侵袭能力（P < 0.01）。
PKC与涎腺腺样囊性癌的侵袭转移密切相关；两种PKC抑制剂显著抑制SACC - 83细胞系细胞的黏附、运动及体外侵袭能力。
