[Dynamic changes in dermal cell cycles, contents of hydroproline and ratios of collagen type I/III in the burned wound].

OBJECTIVE

To study the dynamic changes in the dermal cell cycles contents of hydroproline and ratios of collagen type I/III in the burned wound.

METHODS

The dermal cell cycles, contents of hydroproline, ratios of collagen type I/III and pathologic changes were dynamically observed in 48 deep partial burn rats (TBSA 10%), attempting to investigate the process of deep partial burn wound healing.

RESULTS

The percentage of S-phase reached the highest point on PBD 10. On PBD21, it was still significantly higher than that of normal group. OHP increased gradually after burn, and peaked on PBD14. On PBD21, the level of OHP was still significantly higher than that of normal group. The ratios of collagen type I/III decreased gradually and significantly lower than those of the normal group after burn. The strips of WBC infiltrating into wound were observed on PBD3, 7 and 10. On PBD 14, a large number of regenerative epidermal cells crept over wound surface.

CONCLUSIONS

The inflammation is obvious from PBD3 to PBD10. The proliferation of dermal cell and the synthesis, deposition of new collagen are predominant from PBD10 to PBD14, and the repair of dermis is not complete on PBD21. The proliferation and migration of epidermal cells are major activities from PBD14 to PBD21.