Li Hua-Bin, Lai Jia-Ping, Jiang Yue, Chen Feng
Department of Botany, The University of Hong Kong, China.
J Chromatogr A. 2002 Jan 18;943(2):235-9. doi: 10.1016/s0021-9673(01)01441-8.
High-speed counter-current chromatography was applied to the isolation and purification of salvianolic acid B from the Chinese medicinal plant Salvia miltiorrhiza Bunge. The crude salvianolic acid B was obtained by extraction with ethanol-water from S. miltiorrhiza Bunge. Preparative high-speed counter-current chromatography with a two-phase solvent system composed of n-hexane-ethyl acetate-ethanol-water (3:7:1:9, v/v) was successfully performed yielding 342 mg salvianolic acid B at 98% purity from 500 mg of the crude extract in a one-step separation.
采用高速逆流色谱法从中药丹参中分离纯化丹酚酸B。通过乙醇 - 水提取丹参得到丹酚酸B粗品。以正己烷 - 乙酸乙酯 - 乙醇 - 水（3:7:1:9，v/v）组成的两相溶剂系统进行制备型高速逆流色谱，从500 mg粗提取物中一步分离成功获得342 mg纯度为98%的丹酚酸B。
